Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
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Structure of the miniature type V-F CRISPR-Cas effector enzyme.
Satoru N. Takeda,Ryoya Nakagawa,Sae Okazaki,Hisato Hirano,Kan Kobayashi,Tsukasa Kusakizako,Tomohiro Nishizawa,Keitaro Yamashita,Hiroshi Nishimasu,Osamu Nureki +9 more
TL;DR: Cryo-electron microscopy structure of Cas12f1 (also known as Cas14a) in complex with a guide RNA and its target DNA revealed that two Cas 12f1 molecules assemble with the single guide RNA to recognize the double-stranded DNA target, thereby explaining how the miniature Cas12 f1 enzyme achieves RNA-guided DNA cleavage as an "asymmetric homodimer."
Journal ArticleDOI
Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells.
Yuanming Wang,Yuanming Wang,Kaiwen Ivy Liu,Norfala-Aliah Binte Sutrisnoh,Norfala-Aliah Binte Sutrisnoh,Harini Srinivasan,Harini Srinivasan,Junyi Zhang,Junyi Zhang,Jia Li,Jia Li,Fan Zhang,Charles Richard John Lalith,Heyun Xing,Raghuvaran Shanmugam,Raghuvaran Shanmugam,Jia Nee Foo,Jia Nee Foo,Hwee Ting Yeo,Hwee Ting Yeo,Kean Hean Ooi,Tore Bleckwehl,Yi Yun Rachel Par,Yi Yun Rachel Par,Shi Mun Lee,Shi Mun Lee,Nur Nadiah Binte Ismail,Nur Nadiah Binte Ismail,Nur Aidah Binti Sanwari,Nur Aidah Binti Sanwari,Si Ting Vanessa Lee,Si Ting Vanessa Lee,Jan Lew,Jan Lew,Meng How Tan,Meng How Tan +35 more
TL;DR: This work systematically compares five different CRISPR-Cas systems in human cells by targeting 90 sites in genes with varying expression levels, delineates design parameters for each CRISpr-Cas system and will serve as a useful reference for future genome engineering studies.
Journal ArticleDOI
CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica
TL;DR: The CRISPR-Cpf1 system is optimized and achieved high-editing efficiency for two counter-selectable markers in the industrially-relevant oleaginous yeast Yarrowia lipolytica and the toolkit is expanded to edit three sulfur house-keeping genetic markers, which confers yeast distinct colony color changes due to the formation of PbS (lead sulfide) precipitates.
Journal ArticleDOI
Expanding the Potential of CRISPR-Cpf1-Based Genome Editing Technology in the Cyanobacterium Anabaena PCC 7120
Tian-Cai Niu,Gui-Ming Lin,Li-Rui Xie,Zi-Qian Wang,Wei-Yue Xing,Ju-Yuan Zhang,Cheng-Cai Zhang +6 more
TL;DR: The genome editing vectors and the strategies developed here will expand the ability to study and engineer cyanobacteria, which are extensively used for fundamental studies, biotechnological applications including biofuel production, and synthetic biology research.
Journal ArticleDOI
Robust CRISPR/Cpf1 (Cas12a)-mediated genome editing in allotetraploid cotton (Gossypium hirsutum).
Bo Li,Hangping Rui,Yajun Li,Qiongqiong Wang,Muna Alariqi,Lei Qin,Lin Sun,Xiao Ding,Fuqiu Wang,Jiawei Zou,Yanqing Wang,Daojun Yuan,Xianlong Zhang,Shuangxia Jin,Shuangxia Jin +14 more
TL;DR: This is the first report of the application of the CRISPR/Cpf1 system for cotton genome editing with a very high efficiency (87%) and no off- target effects were detected in the most potential off-target sites.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.