Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs
Fillip Port,Simon L. Bullock +1 more
TL;DR: It is demonstrated that the tRNA–sgRNA system markedly increases the efficacy of conditional gene disruption by Cas9 and can promote editing by the recently discovered RNA-guided endonuclease Cpf1.
Journal ArticleDOI
Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein.
Winston X. Yan,Shaorong Chong,Huaibin Zhang,Kira S. Makarova,Eugene V. Koonin,David R. Cheng,David Scott +6 more
TL;DR: The small size, minimal targeting constraints, and modular regulation of Cas13d effectors further expands the CRISPR toolkit for RNA-manipulation and detection.
Journal ArticleDOI
CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications
TL;DR: An overview of current advances on applications of this technology in plants is presented, emphasizing general considerations for establishment of CRISPR/Cas9 vector platforms, strategies for multiplex editing, methods for analyzing theinduced mutations, factors affecting editing efficiency and specificity, and features of the induced mutations and applications of the CRISpr/ Cas9 system in plants.
Journal ArticleDOI
Engineered Cpf1 variants with altered PAM specificities
Linyi Gao,Linyi Gao,David Benjamin Turitz Cox,David Benjamin Turitz Cox,Winston X. Yan,Winston X. Yan,Winston X. Yan,John C. Manteiga,Martin W. Schneider,Takashi Yamano,Hiroshi Nishimasu,Hiroshi Nishimasu,Osamu Nureki,Nicola Crosetto,Feng Zhang +14 more
TL;DR: A structure-guided mutagenesis screen to increase the targeting range of Cpf1 by approximately threefold in human coding sequences to one cleavage site per ∼11 bp and introduces the identified PAM-interacting mutations at their corresponding positions in LbCpf 1, which altered its PAM specificity.
Journal ArticleDOI
Muscle-specific CRISPR/Cas9 dystrophin gene editing ameliorates pathophysiology in a mouse model for Duchenne muscular dystrophy.
Niclas E. Bengtsson,John K. Hall,Guy L. Odom,Michael Phelps,Colin Andrus,R. David Hawkins,Stephen D. Hauschka,Joel R. Chamberlain,Jeffrey S. Chamberlain +8 more
TL;DR: It is demonstrated that AAV-mediated muscle-specific gene editing has significant potential for therapy of neuromuscular disorders and systemic administration of the vectors results in widespread expression of dystrophin in both skeletal and cardiac muscles.
References
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.