Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Non-viral delivery systems for CRISPR/Cas9-based genome editing: Challenges and opportunities
Ling Li,Shuo Hu,Xiaoyuan Chen +2 more
TL;DR: The pros and cons of delivering CRISPR/Cas9 systems in the form of plasmid, mRNA, or protein are analyzed and promising strategies to overcome barriers are proposed.
Journal ArticleDOI
History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology.
TL;DR: The fascinating history of CRISPR-Cas systems is discussed, from the original observation of an enigmatic sequence in E. coli to genome editing in humans and providing insights into the origin and evolution of this system from mobile genetic elements denoted casposons.
Posted ContentDOI
Protocol: Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening
Julia Joung,Silvana Konermann,Silvana Konermann,Silvana Konermann,Jonathan S. Gootenberg,Omar O. Abudayyeh,Randall Jeffrey Platt,Randall Jeffrey Platt,Randall Jeffrey Platt,Brigham,Brigham,Brigham,Neville E. Sanjana,Neville E. Sanjana,Neville E. Sanjana,Feng Zhang +15 more
TL;DR: A protocol for genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system is described to validate candidate genes identified from the screen and strategies for confirming the screening phenotype as well as genetic perturbation are described.
Journal ArticleDOI
Applying CRISPR/Cas for genome engineering in plants: the best is yet to come
TL;DR: It will be desirable to apply the CRISPR/Cas nuclease system for the mutation of regulatory elements and for more complex genome rearrangements, like transcriptional regulators or DNA modifying enzymes, in the future.
Journal ArticleDOI
Multiplexed CRISPR technologies for gene editing and transcriptional regulation.
TL;DR: This review discusses multiplexed CRISPR technologies and describes methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo, and offers a glimpse of emerging challenges and emphasize experimental considerations for future studies.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
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Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.