Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity
Stefano Stella,Pablo Mesa,Johannes Thomsen,Bijoya Paul,Pablo Alcón,Simon Jensen,Bhargav Saligram,Matias E. Moses,Nikos S. Hatzakis,Guillermo Montoya +9 more
TL;DR: These findings illustrate why Cas12a cuts its target DNA and unleashes unspecific cleavage activity, degrading ssDNA molecules after activation and propose a model whereby the conformational activation of the enzyme results in indiscriminate ssDNA cleavage.
Journal ArticleDOI
CRISPR-Cas9 DNA Base-Editing and Prime-Editing.
TL;DR: The development of various base-editors are described, their technical advantages and limitations are assessed, and their therapeutic potential to treat debilitating human diseases are discussed.
Journal ArticleDOI
Conventional and Molecular Techniques from Simple Breeding to Speed Breeding in Crop Plants: Recent Advances and Future Outlook
Sunny Ahmar,Rafaqat A. Gill,Ki-Hong Jung,Aroosha Faheem,Muhammad Uzair Qasim,Mustansar Mubeen,Weijun Zhou +6 more
TL;DR: Recent findings on several aspects of crop breeding are summarized to describe the evolution of plant breeding practices, from traditional to modern speed breeding combined with genome editing tools, which aim to produce crop generations with desired traits annually.
Journal ArticleDOI
CrispRVariants charts the mutation spectrum of genome engineering experiments.
Helen Lindsay,Alexa Burger,Berthin Biyong,Anastasia Felker,Christopher W. Hess,Jonas Zaugg,Elena Chiavacci,Carolin Anders,Martin Jinek,Christian Mosimann,Mark D. Robinson +10 more
TL;DR: CrispRVariants as discussed by the authors is a R-based toolkit that resolves and localizes individual mutant alleles with respect to the endonuclease cut site and provides insight toward effective guide and amplicon design as well as the mutagenic process.
Journal ArticleDOI
Viral vectors for therapy of neurologic diseases.
Sourav Roy Choudhury,Eloise Hudry,Casey A. Maguire,Miguel Sena-Esteves,Xandra O. Breakefield,Paola Grandi +5 more
TL;DR: An overview of the current state and advances in the field of viral vector-mediated gene therapy for neurological disorders is provided, as well as preclinical and clinical progress made thus far for brain cancer and various neurodegenerative and neurometabolic disorders.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.