Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases
Masato Ohtsuka,Masahiro Sato,Hiromi Miura,Shuji Takabayashi,Makoto Matsuyama,Takayuki Koyano,Naomi Arifin,Shingo Nakamura,Kenta Wada,Channabasavaiah B. Gurumurthy +9 more
TL;DR: A robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation is presented.
Journal ArticleDOI
Type III CRISPR-Cas Immunity: Major Differences Brushed Aside.
TL;DR: It is found that the Type III CRISPR-Cas systems are both RNases and target RNA-activated DNA nucleases, and the base-pairing potential between the target RNA and theCRISPR RNA 5'-handle seems to play an important role in discriminating self and non-self nucleic acids.
Journal ArticleDOI
Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay.
TL;DR: A novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections was described and the practical applicability was successfully demonstrated through the detection of emerging red-blotch viral infections in grapevine samples collected from commercial vineyards.
Journal ArticleDOI
Patterns of CRISPR/Cas9 activity in plants, animals and microbes
Luisa Bortesi,Changfu Zhu,Julia Zischewski,Lucia Perez,Ludovic Bassie,Riad Nadi,Giobbe Forni,Sarah Boyd Lade,Erika Soto,Xin Jin,Vicente Medina,Gemma Villorbina,Pilar Muñoz,Gemma Farré,Rainer Fischer,Rainer Fischer,Richard M. Twyman,Teresa Capell,Paul Christou,Stefan Schillberg +19 more
TL;DR: Critically analyse the activity of CRISPR/Cas9 and related systems in different plant species and compare the outcomes in animals and microbes to draw broad conclusions about the design principles required for effective genome editing in different organisms.
Journal ArticleDOI
Design and analysis of CRISPR-Cas experiments.
Ruth E Hanna,John G. Doench +1 more
TL;DR: The computational methods and tools that have become indispensable for planning and analyzing CRISPR experiments are reviewed and actively maintained so that researchers across disciplines can rely on a stable set of excellent computational resources forCRISPR–Cas experiments.
References
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
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