Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
More filters
Journal ArticleDOI
Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing.
Wakako Kumita,Kenya Sato,Yasuhiro Suzuki,Yoko Kurotaki,Takeshi Harada,Takeshi Harada,Yang Zhou,Noriyuki Kishi,Kengo Sato,Atsu Aiba,Yasubumi Sakakibara,Guoping Feng,Hideyuki Okano,Erika Sasaki,Erika Sasaki +14 more
TL;DR: Results indicated that CRISPR/Cas9 may be utilized to produce KO/KI marmosets via gene editing, and further improvement of KI strategies is required.
Journal ArticleDOI
Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)
TL;DR: In this paper , a CRISPR-Cas12a-based E-CRISPR DNA-sensor was used for early detection of virus DNA in clinical samples, contaminated foods, soil or water, and reducing the socioeconomic impact of diseases such as COVID-19.
Journal ArticleDOI
The Future of Multiplexed Eukaryotic Genome Engineering.
David B. Thompson,Soufiane Aboulhouda,Soufiane Aboulhouda,Eriona Hysolli,Eriona Hysolli,Cory Smith,Cory Smith,Stan Wang,Stan Wang,Oscar Castanon,Oscar Castanon,Oscar Castanon,George M. Church,George M. Church +13 more
TL;DR: A broad look at future directions is offered, covering emergent CRISPR-based technologies, advances in intracellular delivery, and new DNA assembly approaches that may enable future genome editing on a massively multiplexed scale.
Journal ArticleDOI
CRISPR diagnostics
TL;DR: Jiao et al. as discussed by the authors proposed a new CRISPR-based tool to distinguish several SARS-CoV-2 variants in a single reaction, and used it for the detection of the SARS CoV2 RNA genome.
Journal ArticleDOI
Advancing sensing technology with CRISPR: From the detection of nucleic acids to a broad range of analytes - A review.
TL;DR: This review summarized the strategies of CRISPR biosensing for non-nucleic-acid analytes, the latest development of nucleic acid detection, and proposed the challenges and outlook ofCRISPR-powered biosensors.
References
More filters
Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
Journal ArticleDOI
MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.