Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Book ChapterDOI
RNA Therapeutics: How Far Have We Gone?
TL;DR: These approaches and their translation into clinics are reviewed to give a brief overview on the difficulties to its application as well as the research that is being done to overcome them.
Journal ArticleDOI
Regulation of the RNA and DNA nuclease activities required for Pyrococcus furiosus Type III-B CRISPR–Cas immunity
TL;DR: This work describes how the RNase and DNase activities associated with Type III-B immunity in Pyrococcus furiosus (Pfu) are regulated by target RNA features and second messenger signaling events, and highlights the complex regulatory mechanisms controlling Type III CRISPR immunity.
Journal ArticleDOI
CRISPR-Cas systems in multicellular cyanobacteria.
Shengwei Hou,Manuel Brenes-Álvarez,Viktoria Reimann,Omer S. Alkhnbashi,Rolf Backofen,Alicia M. Muro-Pastor,Wolfgang R. Hess +6 more
TL;DR: The diversity and presence of numerous CRISPR-Cas systems in DNA elements that are programmed for homologous recombination make filamentous cyanobacteria a prolific resource for their study.
Journal ArticleDOI
Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9.
Xiya Zhang,Puping Liang,Chenhui Ding,Zhen Zhang,Jianwen Zhou,Xiaowei Xie,Rui Huang,Ying Sun,Hongwei Sun,Jinran Zhang,Yanwen Xu,Zhou Songyang,Junjiu Huang +12 more
TL;DR: SaCas9 can specifically cleave the target gene locus, leading to successful gene knock-out and precise knock-in in mouse zygotes, and highlight the potential of using SaCas9 for genome editing in preimplantation embryos and producing gene-modified animal models.
Journal ArticleDOI
Enhancing Protein Production Yield from Chinese Hamster Ovary Cells by CRISPR Interference.
TL;DR: The application of CRISPRi in CHO cells to enhance recombinant protein production and may pave a new avenue to CHO cell engineering is demonstrated for the first time.
References
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.