Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Journal ArticleDOI
CRISPR-Cas12a-assisted nucleic acid detection.
Shi-Yuan Li,Qiu-Xiang Cheng,Jingman Wang,Xiao-Yan Li,Zilong Zhang,Song Gao,Rui-Bing Cao,Guoping Zhao,Guoping Zhao,Jin Wang +9 more
TL;DR: In a recent study, it was found that Cas 12a, which belongs to the class 2 type V-A CRISPR-Cas system, performed collateral cleavage on non-targeted ssDNAs upon the formation of the Cas12a/crRNA/target DNA ternary complex.
Journal ArticleDOI
The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
TL;DR: It is shown that type V-A Cpf1 from Francisella novicida is a dual-nuclease that is specific to crRNA biogenesis and target DNA interference and constitutes the most minimalistic of the CRISPR–Cas systems so far described.
Journal ArticleDOI
Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
Silvana Konermann,Peter Lotfy,Nicholas J. Brideau,Jennifer Oki,Maxim N. Shokhirev,Patrick D. Hsu +5 more
TL;DR: This work identifies an uncharacterized family of RNA-guided, RNA-targeting CRISPR systems that is classified as type VI-D, and presents CasRx as a programmable RNA-binding module for efficient targeting of cellular RNA, enabling a general platform for transcriptome engineering and future therapeutic development.
Journal ArticleDOI
Applications of CRISPR technologies in research and beyond
TL;DR: Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms and is being used to expedite crop and livestock breeding, engineer new antimicrobials and control disease- carrying insects with gene drives.
Journal ArticleDOI
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array
Bernd Zetsche,Matthias Heidenreich,Prarthana Mohanraju,Iana Fedorova,Jeroen Kneppers,Jeroen Kneppers,Ellen M DeGennaro,Ellen M DeGennaro,Nerges Winblad,Sourav R Choudhury,Omar O. Abudayyeh,Jonathan S. Gootenberg,Wen Y Wu,David A. Scott,David A. Scott,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,John van der Oost,Feng Zhang +19 more
TL;DR: It is shown that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing.
References
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TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
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