Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Optimization of AsCas12a for combinatorial genetic screens in human cells
Peter C DeWeirdt,Kendall R Sanson,Annabel K Sangree,Mudra Hegde,Ruth E Hanna,Marissa N Feeley,Audrey L Griffith,Teng Teng,Samantha M Borys,Christine Strand,J. Keith Joung,Benjamin P. Kleinstiver,Xuewen Pan,Alan Huang,John G. Doench +14 more
TL;DR: Improved Cas12a variants and sgRNA design rules enhance genome-wide screens and are validated by screening for synthetic lethalities in OVCAR8 and A375 cancer cells, discovering an interaction between MARCH5 and WSB2.
Journal ArticleDOI
Cas9, Cpf1 and C2c1/2/3―What's next?
TL;DR: Various improvements and alternatives of CRISPR-Cas systems are described, including engineered Cas9 variants, Cas9 homologs, and novel Cas proteins other than Cas9 that enable flexible genome engineering with high efficiency and specificity, orthogonal genetic control at multiple gene loci, gene knockdown, or fluorescence imaging of transcripts mediated by RNA targeting, and beyond.
Journal ArticleDOI
Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems
TL;DR: In this article, a simplified single transcriptional unit (SSTU) CRISPR system was developed for multiplex gene editing in rice using FnCpf1, LbcPf1 or Cas9.
Journal ArticleDOI
Heavily and Fully Modified RNAs Guide Efficient SpyCas9-mediated Genome Editing
Aamir Mir,Julia F. Alterman,Matthew R. Hassler,Alexandre J. Debacker,Edward Hudgens,Dimas Echeverria,Michael H. Brodsky,Anastasia Khvorova,Jonathan K. Watts,Erik J. Sontheimer +9 more
TL;DR: Modifications at all positions of the crRNA guide and tracrRNA cofactor are explored, and modified versions that are more potent and stable than their unmodified counterparts in editing human cells are identified.
Journal ArticleDOI
Genome editing in cardiovascular diseases
Alanna Strong,Kiran Musunuru +1 more
TL;DR: The applications of genome-EDiting technology throughout cardiovascular disease research and the prospect of in vivo genome-editing therapies in the future are discussed.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.