Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage
A. Nahmad,Eli Reuveni,Ella Goldschmidt,Tamar Tenne,Meytal Liberman,Miryam Horovitz-Fried,Rami Khosravi,Hila Kobo,Eyal Reinstein,Asaf Madi,Uri Ben-David,Adi Barzel +11 more
TL;DR: In this article , the authors investigated genome editing outcomes in primary human T cells transfected with CRISPR-Cas9 and guide RNAs targeting genes for TCR chains and programmed cell death protein.
Journal ArticleDOI
CRISPR-Cas9 genome engineering: Treating inherited retinal degeneration
Erin R Burnight,Joseph C. Giacalone,Jessica A. Cooke,Jessica R. Thompson,Laura R. Bohrer,Kathleen R. Chirco,Arlene V. Drack,John H. Fingert,Kristan S. Worthington,Luke A Wiley,Robert F. Mullins,Edwin M. Stone,Budd A. Tucker +12 more
TL;DR: The advent of induced pluripotent stem cell and CRISPR‐Cas9 gene editing technologies affords researchers and clinicians a powerful means by which to develop strategies to treat patients with inherited retinal dystrophies.
Journal ArticleDOI
CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1.
Mihris Ibnu Saleem Naduthodi,Prarthana Mohanraju,Christian Südfeld,Sarah D'Adamo,Maria J. Barbosa,John van der Oost +5 more
TL;DR: It is shown that the delivery of Cas RNP by electroporation can be an alternative approach to the presently reported plasmid-based Cas9 method for generating mutants of N. oceanica and can be used for efficient generation of targeted mutants in Nannochloropsis sp.
Journal ArticleDOI
Beyond Native Cas9: Manipulating Genomic Information and Function
TL;DR: The discovery of a variety of Cas9 orthologs and engineered variants enables high-fidelity genome editing and a wider selection of genomic targets, andCRISPR-mediated deaminases enable more precise and predictable genome editing compared with CRISPR nuclease-based editing.
Journal ArticleDOI
CRISPR-Cas Systems and the Paradox of Self-Targeting Spacers.
Franziska Wimmer,Chase L. Beisel +1 more
TL;DR: The known frequency of self-targeting spacers in natural CRISPR-Cas systems, how these spacers can be incorporated intoCRISPR arrays, and how the host can evade lethal attack are discussed.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.