Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
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Patent
Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
Feng Zhang,Chie-yu Lin,Fei Ran +2 more
TL;DR: In this article, the authors provide delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences, including vectors and vector systems, some of which encode one or more components of a CRISPR complex.
Journal ArticleDOI
Animal Models of Hypertension: A Scientific Statement From the American Heart Association
Lilach O. Lerman,Theodore W. Kurtz,Rhian M. Touyz,David H. Ellison,Alejandro R. Chade,Steven D. Crowley,David L. Mattson,John J. Mullins,Jeffrey L. Osborn,Alfonso Eirin,Jane F. Reckelhoff,Costantino Iadecola,Thomas M. Coffman +12 more
TL;DR: The utility of animal models for improving the understanding of the pathogenesis, prevention, and treatment of hypertension and its comorbidities depends on their validity for representing human forms of hypertension, including responses to therapy, and on the quality of studies in those models.
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C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism
TL;DR: The findings indicate that sgRNA assembly is achieved through a mechanism distinct from that reported previously for Cas9 or Cpf1 endonucleases, which renders C2c1 a valuable addition to the current arsenal of genome-editing tools.
Journal ArticleDOI
Viral Delivery Systems for CRISPR.
TL;DR: The history and recent development of CRISPR and viral vectors are reviewed and their applications as a powerful tool in furthering the exploration of disease mechanisms and therapies are discussed.
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dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression.
Henriette O'Geen,Chonghua Ren,Chonghua Ren,Charles M Nicolet,Andrew A. Perez,Julian Halmai,Victoria M. Le,Joel P. Mackay,Peggy J. Farnham,David J. Segal +9 more
TL;DR: The results suggest that so-called repressive histone modifications are not sufficient for gene repression, and the easily programmable dCas9 toolkit allowed precise control of epigenetic information and dissection of the relationship between the epigenome and gene regulation.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.