Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
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Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia.
Sateesh Krishnamurthy,Christine L. Wohlford-Lenane,Suhas Kandimalla,Gilles Sartre,David K. Meyerholz,Vanessa Théberge,Stéphanie Hallée,Anne-Marie Duperré,Thomas Del’Guidice,Jean-Pascal Lepetit-Stoffaes,Xavier Barbeau,David Guay,Paul B. McCray +12 more
TL;DR: E engineered amphiphilic peptides are used to shuttle proteins and CRISPR RNPs into airway cells in vivo, advancing potential therapeutic avenues for protein and Cas RNP delivery to refractory airway epithelial cells.
Journal ArticleDOI
Editing the microbiome the CRISPR way.
TL;DR: Different types of CRISPR-Cas systems can be used to modify the genome of gut microorganisms and bacteriophages and present new avenues for the development of drugs that target the microbiome.
Journal ArticleDOI
Hit and go CAS9 delivered through a lentiviral based self-limiting circuit
Gianluca Petris,Antonio Casini,Claudia Montagna,Francesca Lorenzin,Davide Prandi,Alessandro Romanel,Jacopo Zasso,Luciano Conti,Francesca Demichelis,Anna Cereseto +9 more
TL;DR: A Self-Limiting Cas9 circuit for Enhanced Safety and specificity (SLiCES) which consists of an expression unit for Streptococcus pyogenes Cas9, a self-targeting sgRNA and a second sg RNA targeting a chosen genomic locus results in increased genome editing specificity by controlling Cas9 levels.
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Molecular biology at the cutting edge: A review on CRISPR/CAS9 gene editing for undergraduates
TL;DR: The goals of this review are to explain how CRISPR functions as a prokaryotic immune system, describe how researchers generate mutations withCRISPR/Cas9, highlight how Cas9 has been adapted for new functions, and discuss ethical considerations of genome editing.
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xCas9 expands the scope of genome editing with reduced efficiency in rice.
TL;DR: This data indicates that Cas9-sgRNA complexes will directly eject the DNA template if no PAMs are recognized before base pairing between target sites and sgRNA (Sternberg et al., 2014).
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.