Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
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Enhancement of target specificity of CRISPR-Cas12a by using a chimeric DNA-RNA guide
Hanseop Kim,Hanseop Kim,Wi Jae Lee,Wi Jae Lee,Yeounsun Oh,Yeounsun Oh,Seung Hun Kang,Seung Hun Kang,Junho K. Hur,Junho K. Hur,Hyomin Lee,Woo Jeung Song,Kyung Seob Lim,Young-Ho Park,Bong Seok Song,Yeung Bae Jin,Bong-Hyun Jun,Cheulhee Jung,Dong-Seok Lee,Sun-Uk Kim,Sun-Uk Kim,Seung-Hwan Lee +21 more
TL;DR: A CRISPR–Cas12a that can induce mutations in target DNA sequences in a highly specific and effective manner by partially substituting the (cr)RNA with DNA to change the energy potential of base pairing to the target DNA is developed.
Journal ArticleDOI
The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead
TL;DR: Novel functional genomics approaches with potential to overcome some of the drawbacks of the current strategies are discussed, and progress and challenges in the deployment of these approaches in the control of CMGs are discussed.
Journal ArticleDOI
Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.
Caitlin A. Nichols,Caitlin A. Nichols,William J. Gibson,William J. Gibson,Meredith S. Brown,Jack A. Kosmicki,John P. Busanovich,Hope Wei,Laura M. Urbanski,Naomi Curimjee,Ashton C. Berger,Ashton C. Berger,Galen F. Gao,Andrew D. Cherniack,Andrew D. Cherniack,Sirano Dhe-Paganon,Brenton R. Paolella,Rameen Beroukhim +17 more
TL;DR: This work identifies 5664 variants in 1278 essential genes that undergo LOH in cancer and evaluated the potential for each to be targeted using allele-specific gene-editing, RNAi, or small-molecule approaches.
Patent
Compositions and methods for in vivo excision of hiv-1 proviral dna
TL;DR: In this paper, methods and kits for excising HIV-1 DNA in vivo are provided, which employ Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-Associated (cas) proteins.
Journal ArticleDOI
Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9
Daniel Agudelo,Sophie Carter,Minja Velimirovic,Alexis Duringer,Jean-François Rivest,Sébastien Lévesque,Jeremy Loehr,Mathilde Mouchiroud,Denis Cyr,Paula J. Waters,Mathieu Laplante,Sylvain Moineau,Adeline Goulet,Yannick Doyon +13 more
TL;DR: It is found that St1Cas9 strain variants enable targeting to five distinct A-rich PAMs and provide a structural basis for their specificities.
References
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Multiplex Genome Engineering Using CRISPR/Cas Systems
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