Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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In vivo high-throughput profiling of CRISPR-Cpf1 activity
Hui K Kim,Myungjae Song,Myungjae Song,Jinu Lee,A. Vipin Menon,Soobin Jung,Young Mook Kang,Jae W Choi,Eui-Jeon Woo,Hyun Chul Koh,Jin Wu Nam,Hyongbum Kim +11 more
TL;DR: This work developed a method for evaluating Cpf1 activity, based on target sequence composition in mammalian cells, in a high-throughput manner, and defined target-sequence-dependent activity profiles of AsCpf 1, which enabled the development of a web tool that predicts the indel frequencies for given target sequences.
Journal ArticleDOI
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules.
Mindong Liang,Mindong Liang,Zilong Li,Weishan Wang,Jiakun Liu,Jiakun Liu,Leshi Liu,Guoliang Zhu,Loganathan Karthik,Man Wang,Ke-Feng Wang,Zhong Wang,Jing Yu,Yuting Shuai,Jiaming Yu,Lu Zhang,Zhiheng Yang,Chuan Li,Qian Zhang,Tong Shi,Liming Zhou,Feng Xie,Huanqin Dai,Xueting Liu,Jie Zhang,Guang Liu,Ying Zhuo,Buchang Zhang,Chenli Liu,Shanshan Li,Xuekui Xia,Yaojun Tong,Yanwen Liu,Gil Alterovitz,Gao-Yi Tan,Lixin Zhang +35 more
TL;DR: CaT-SMelor is presented which uses Cas12a and allosteric transcription factors to detect small molecules in the nanomolar range and directly measured the uric acid concentration in clinical human blood samples, indicating a great potential of CaT- SMelor in the detection of small molecules.
Journal ArticleDOI
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators
TL;DR: This work provides a robust CRISPR–dCas9-based platform for enacting complex transcription programs that is suitable for large-scale transcriptome engineering and devised AND, OR, NAND, and NOR dCas9 logic operators and a diametric regulator that activates gene expression with one inducer and represses with another.
Journal ArticleDOI
Advances in genome editing through control of DNA repair pathways.
TL;DR: This Review discusses the DNA repair pathways that underlie genome editing and recent improvements and strategies to yield desired genomic alterations in cells and organisms.
Journal ArticleDOI
The emerging and uncultivated potential of CRISPR technology in plant science.
TL;DR: A Review informatively summaries the recent development and breakthroughs of CRISPR technology, with a focus on progresses, challenges and potential utility in plant science.
References
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Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
Journal ArticleDOI
MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.