Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize.
Keunsub Lee,Yingxiao Zhang,Benjamin P. Kleinstiver,Jimmy A. Guo,Martin J. Aryee,Jonah Miller,Aimee Malzahn,Scott Zarecor,Carolyn J. Lawrence-Dill,J. Keith Joung,Yiping Qi,Kan Wang +11 more
TL;DR: The results suggest that the CRISPR/Cas9 system used in this study is highly efficient and specific for genome editing in maize, while CRISpr/Cas12a needs further optimization for improved editing efficiency.
Patent
Optimized CRISPR-Cas double nickase systems, methods and compositions for sequence manipulation
TL;DR: In this article, the authors provide delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences, including vectors and vector systems, some of which encode one or more components of a CRISPR complex.
Journal ArticleDOI
Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.
TL;DR: In this article, crystal structures of the VQR, EQR, and VRER SpCas9 variants bound to target DNAs containing their preferred NAAG PAM sequences were reported.
Journal ArticleDOI
How a fungus shapes biotechnology: 100 years of Aspergillus niger research.
TL;DR: This review assesses main developments in the field of A. niger biology over the last 100 years and highlights scientific breakthroughs and discoveries which were influential for both basic and applied fungal research in and outside the A.Niger community.
Journal ArticleDOI
Advances in Industrial Biotechnology Using CRISPR-Cas Systems.
TL;DR: The fundamental characteristics of CRISPR-Cas systems are explored and how these features can be used in industrial settings are highlighted.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
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TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.