Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts.
Carlo Cosimo Campa,Niels R. Weisbach,António J. Santinha,Danny Incarnato,Randall Jeffrey Platt,Randall Jeffrey Platt +5 more
TL;DR: A single transcript encoding Cas12a and an array of CRISPR RNAs enables multiplexed genome engineering, from multiple knockouts to transcriptional activation or repression to orthogonal transcriptional control and editing in the same sample.
Journal ArticleDOI
Review of CRISPR/Cas9 sgRNA Design Tools.
TL;DR: Various sgRNA design tools are reviewed, mainly focusing on their on-target efficiency prediction model and off-target detection algorithm.
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Repurposing CRISPR-Cas12b for mammalian genome engineering.
Fei Teng,Tongtong Cui,Guihai Feng,Lu Guo,Kai Xu,Qingqin Gao,Tianda Li,Jing Li,Qi Zhou,Wei Li +9 more
TL;DR: The identification of a Cas12b system from the Alicyclobacillus acidiphilus (AaCas12b), which maintains optimal nuclease activity over a wide temperature range (31 °C–59 °C), establishes CRISPR-Cas 12b as a versatile tool for mammalian genome engineering.
Journal ArticleDOI
The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit
TL;DR: The current mechanistic understanding of newly discovered single-protein Cas endonucleases is reviewed, underscoring the phylogenetic divergence of related CRISPR-Cas systems.
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Enhancing genetic gain in the era of molecular breeding
Yunbi Xu,Ping Li,Cheng Zou,Yanli Lu,Chuanxiao Xie,Xuecai Zhang,Boddupalli M. Prasanna,Michael Olsen +7 more
TL;DR: All the strategies can be integrated with other widely used conventional approaches in breeding programs to enhance genetic gain and more transdisciplinary approaches, team breeding, will be required to address the challenge of maintaining a plentiful and safe food supply for future generations.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.