Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment
Juliana Durack,Susan V. Lynch,Snehal Nariya,Nirav R. Bhakta,Avraham Beigelman,Mario Castro,Anne Marie Dyer,Elliot Israel,Monica Kraft,Richard J. Martin,David T. Mauger,Sharon R. Rosenberg,Tonya Sharp-King,Steven R. White,Prescott G. Woodruff,Pedro C. Avila,Loren C. Denlinger,Fernando Holguin,Stephen C. Lazarus,Njira L Lugogo,Wendy C. Moore,Stephen P. Peters,Loretta G. Que,Lewis J. Smith,Christine A. Sorkness,Michael E. Wechsler,Sally E. Wenzel,Homer A. Boushey,Yvonne J. Huang +28 more
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TLDR
Even in subjects with mild steroid‐naive asthma, differences in the bronchial microbiome are associated with immunologic and clinical features of the disease, suggesting possible microbiome targets for future approaches to asthma treatment or prevention.Abstract:
Background Compositional differences in the bronchial bacterial microbiota have been associated with asthma, but it remains unclear whether the findings are attributable to asthma, to aeroallergen sensitization, or to inhaled corticosteroid treatment. Objectives We sought to compare the bronchial bacterial microbiota in adults with steroid-naive atopic asthma, subjects with atopy but no asthma, and nonatopic healthy control subjects and to determine relationships of the bronchial microbiota to phenotypic features of asthma. Methods Bacterial communities in protected bronchial brushings from 42 atopic asthmatic subjects, 21 subjects with atopy but no asthma, and 21 healthy control subjects were profiled by using 16S rRNA gene sequencing. Bacterial composition and community-level functions inferred from sequence profiles were analyzed for between-group differences. Associations with clinical and inflammatory variables were examined, including markers of type 2–related inflammation and change in airway hyperresponsiveness after 6 weeks of fluticasone treatment. Results The bronchial microbiome differed significantly among the 3 groups. Asthmatic subjects were uniquely enriched in members of the Haemophilus , Neisseria , Fusobacterium , and Porphyromonas species and the Sphingomonodaceae family and depleted in members of the Mogibacteriaceae family and Lactobacillales order. Asthma-associated differences in predicted bacterial functions included involvement of amino acid and short-chain fatty acid metabolism pathways. Subjects with type 2–high asthma harbored significantly lower bronchial bacterial burden. Distinct changes in specific microbiota members were seen after fluticasone treatment. Steroid responsiveness was linked to differences in baseline compositional and functional features of the bacterial microbiome. Conclusion Even in subjects with mild steroid-naive asthma, differences in the bronchial microbiome are associated with immunologic and clinical features of the disease. The specific differences identified suggest possible microbiome targets for future approaches to asthma treatment or prevention.read more
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Asthma and the microbiome
C. Barnig,C. Martin +1 more
TL;DR: The implication of the microbiome in the pathogenesis of human asthma seems to be more and more likely and could have possible therapeutic implications, notably the restoration of a healthy microbiome.
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Aerodigestive dysbiosis in children with chronic cough.
Mikhail Kazachkov,Bianca Kapoor,Patrick Malecha,Benjamin G. Wu,Yonghua Li,Jeremiah Levine,Jessica Erkman,Kathryn Fitzgerald,Libia Moy,Leopoldo N. Segal +9 more
TL;DR: It is hypothesize that in subjects with chronic cough, clinical diagnosis will correlate with distinct microbial signatures detected using culture‐independent methods.
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Application of an ecology-based analytic approach to discriminate signal and noise in low-biomass microbiome studies: whole lung tissue is the preferred sampling method for amplicon-based characterization of murine lung microbiota
Jennifer M. Baker,Kevin J. Hinkle,Roderick A. McDonald,Nicole R. Falkowski,Gary B. Huffnagle,Robert P. Dickson +5 more
TL;DR: In this article, the authors compared bacterial DNA from the lungs of healthy adult mice collected via two common sampling approaches: homogenized whole lung tissue and bronchoalveolar lavage (BAL) fluid, quantified bacterial DNA using droplet digital PCR, characterized bacterial communities using 16S rRNA gene sequencing, and systematically assessed the quantity and identity of bacterial DNA in both specimen types.
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Identifying Bacterial Airways Infection in Stable Severe Asthma Using Oxford Nanopore Sequencing Technologies
M Jabeen,Nicholas D Sanderson,Dona Foster,Derrick W. Crook,Jennifer L Cane,Catherine Borg,Clare Connolly,Samantha Thulborn,Ian D. Pavord,Paul Klenerman,Teresa L Street,Timothy S. C. Hinks +11 more
TL;DR: Haemophilus influenzae was a dominant bacterium in the airways in people with severe asthma and was identified reliably using metagenomic sequencing using an optimized method for Illumina MiSeq and Oxford Nanopore.
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Microbiota: A Missing Link in The Pathogenesis of Chronic Lung Inflammatory Diseases.
TL;DR: In this article, the authors explored the role of the human microbiota in the area of chronic pulmonary diseases, especially COPD, asthma, and CF, and found that the presence of lung microbiota can shape the pathogenic processes underlying chronic inflammation, typically observed in the course of the diseases.
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