Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment
Juliana Durack,Susan V. Lynch,Snehal Nariya,Nirav R. Bhakta,Avraham Beigelman,Mario Castro,Anne Marie Dyer,Elliot Israel,Monica Kraft,Richard J. Martin,David T. Mauger,Sharon R. Rosenberg,Tonya Sharp-King,Steven R. White,Prescott G. Woodruff,Pedro C. Avila,Loren C. Denlinger,Fernando Holguin,Stephen C. Lazarus,Njira L Lugogo,Wendy C. Moore,Stephen P. Peters,Loretta G. Que,Lewis J. Smith,Christine A. Sorkness,Michael E. Wechsler,Sally E. Wenzel,Homer A. Boushey,Yvonne J. Huang +28 more
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TLDR
Even in subjects with mild steroid‐naive asthma, differences in the bronchial microbiome are associated with immunologic and clinical features of the disease, suggesting possible microbiome targets for future approaches to asthma treatment or prevention.Abstract:
Background Compositional differences in the bronchial bacterial microbiota have been associated with asthma, but it remains unclear whether the findings are attributable to asthma, to aeroallergen sensitization, or to inhaled corticosteroid treatment. Objectives We sought to compare the bronchial bacterial microbiota in adults with steroid-naive atopic asthma, subjects with atopy but no asthma, and nonatopic healthy control subjects and to determine relationships of the bronchial microbiota to phenotypic features of asthma. Methods Bacterial communities in protected bronchial brushings from 42 atopic asthmatic subjects, 21 subjects with atopy but no asthma, and 21 healthy control subjects were profiled by using 16S rRNA gene sequencing. Bacterial composition and community-level functions inferred from sequence profiles were analyzed for between-group differences. Associations with clinical and inflammatory variables were examined, including markers of type 2–related inflammation and change in airway hyperresponsiveness after 6 weeks of fluticasone treatment. Results The bronchial microbiome differed significantly among the 3 groups. Asthmatic subjects were uniquely enriched in members of the Haemophilus , Neisseria , Fusobacterium , and Porphyromonas species and the Sphingomonodaceae family and depleted in members of the Mogibacteriaceae family and Lactobacillales order. Asthma-associated differences in predicted bacterial functions included involvement of amino acid and short-chain fatty acid metabolism pathways. Subjects with type 2–high asthma harbored significantly lower bronchial bacterial burden. Distinct changes in specific microbiota members were seen after fluticasone treatment. Steroid responsiveness was linked to differences in baseline compositional and functional features of the bacterial microbiome. Conclusion Even in subjects with mild steroid-naive asthma, differences in the bronchial microbiome are associated with immunologic and clinical features of the disease. The specific differences identified suggest possible microbiome targets for future approaches to asthma treatment or prevention.read more
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Bacterial biogeography of adult airways in atopic asthma.
Juliana Durack,Yvonne J. Huang,Snehal Nariya,Laura S. Christian,K. Mark Ansel,Avraham Beigelman,Mario Castro,Anne-Marie Dyer,Elliot Israel,Monica Kraft,Richard J. Martin,David T. Mauger,Sharon R. Rosenberg,Tonya S. King,Steven R. White,Loren C. Denlinger,Fernando Holguin,Stephen C. Lazarus,Njira L Lugogo,Stephen P. Peters,Lewis J. Smith,Michael E. Wechsler,Susan V. Lynch,Homer A. Boushey +23 more
TL;DR: Induced sputum is superior to nasal brush or oral wash for assessing bronchial microbiota composition in asthmatic adults, and the microbiota in induced spUTum are distinct, reflecting enrichment of oral bacteria.
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TL;DR: To date there is no direct evidence from human or animal studies on the role of lung microbiome in modifying COVID-19 disease; however, related studies support that microbiome can play an essential role in developing immunity against viral infections.
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Phenotypic and genetic aspects of epithelial barrier function in asthmatic patients.
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TL;DR: The ability of the bronchial epithelium to control the balance of inhibitory and activating signals is essential for orchestrating appropriate inflammatory and immune responses and for temporally modulating these responses to limit tissue injury and control the resolution of inflammation during tissue repair.
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Zhang Wang,Nicholas Locantore,Koirobi Haldar,Mohammadali Yavari Ramsheh,Augusta S Beech,Wei Ma,James R Brown,Ruth Tal-Singer,Michael R. Barer,Mona Bafadhel,Gavin C. Donaldson,Jadwiga A. Wedzicha,D. Singh,Tom Wilkinson,Bruce E. Miller,C E Brightling +15 more
TL;DR: The airway microbiome can stratify neutrophilic COPD into subgroups that justify different therapies, and monitoring temporal variability of the airways microbiome may track patient inflammatory status over time.
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Associations between fungal and bacterial microbiota of airways and asthma endotypes
Anukriti Sharma,Anukriti Sharma,Anukriti Sharma,Bharathi Laxman,Edward T. Naureckas,D. Kyle Hogarth,Anne I. Sperling,Julian Solway,Carole Ober,Jack A. Gilbert,Jack A. Gilbert,Jack A. Gilbert,Steven R. White +12 more
TL;DR: This study demonstrates clear differences in the bacterial and fungal microbiota in asthma-associated phenotypes and provides additional support for considering microbial signatures in delineating asthma phenotypes.
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