Journal ArticleDOI
Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: a new approach for drug delivery.
Luisa Pascucci,Valentina Coccè,Arianna Bonomi,Diletta Ami,Piero Ceccarelli,Emilio Ciusani,Lucia Viganò,Alberta Locatelli,Francesca Sisto,Silvia Maria Doglia,Eugenio Parati,Maria Ester Bernardo,Maurizio Muraca,Giulio Alessandri,Gianpietro Bondiolotti,Augusto Pessina +15 more
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TLDR
This work has found that MSCs can acquire strong anti-tumor activity after priming with Paclitaxel (PTX) through their capacity to uptake and then release the drug, suggesting the possibility of using M SCs as a factory to develop drugs with a higher cell-target specificity.About:
This article is published in Journal of Controlled Release.The article was published on 2014-10-28. It has received 644 citations till now.read more
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Exosomes: biogenesis, biologic function and clinical potential
TL;DR: The potential use of exosomes as valuable diagnostic and prognostic biomarkers for their cell-lineage and state-specific contents, and possibilities as therapeutic vehicles for drug and gene delivery are focused on.
Journal ArticleDOI
Applying extracellular vesicles based therapeutics in clinical trials - an ISEV position paper
Thomas Lener,Mario Gimona,Ludwig Aigner,Verena Börger,Edit I. Buzás,Giovanni Camussi,Nathalie Chaput,Devasis Chatterjee,Felipe A. Court,Hernando A. del Portillo,Lorraine O'Driscoll,Stefano Fais,Juan M. Falcón-Pérez,Ursula Felderhoff-Mueser,Lorenzo Fraile,Yong Song Gho,André Görgens,Ramesh C. Gupta,An Hendrix,Dirk M. Hermann,Andrew F. Hill,Fred H. Hochberg,Peter A. Horn,Dominique P.V. de Kleijn,Lambros Kordelas,Boris W. Kramer,Eva-Maria Krämer-Albers,Sandra Laner-Plamberger,Saara Laitinen,Tommaso Leonardi,Magdalena J. Lorenowicz,Sai Kiang Lim,Jan Lötvall,Casey A. Maguire,Antonio Marcilla,Irina Nazarenko,Takahiro Ochiya,Tushar Patel,Shona Pedersen,Gabriella Pocsfalvi,Stefano Pluchino,Peter J. Quesenberry,Ilona G. Reischl,Francisco J. Rivera,Ralf Sanzenbacher,Katharina Schallmoser,Ineke Slaper-Cortenbach,Dirk Strunk,Torsten Tonn,Pieter Vader,Pieter Vader,Bas W.M. van Balkom,Marca H. M. Wauben,Samir El Andaloussi,Samir El Andaloussi,Clotilde Théry,Eva Rohde,Bernd Giebel +57 more
TL;DR: In this paper, the authors summarize recent developments and the current knowledge of extracellular vesicles (EVs) and discuss safety and regulatory requirements that must be considered for pharmaceutical manufacturing and clinical application.
Journal ArticleDOI
Extracellular vesicles in cancer - implications for future improvements in cancer care.
TL;DR: A review of the biophysical properties and physiological functions of extracellular vesicles, particularly their pro-metastatic effects, and highlight the utility of EVs for the development of cancer diagnostics and therapeutics can be found in this paper.
Journal ArticleDOI
Development of exosome-encapsulated paclitaxel to overcome MDR in cancer cells
Myung Soo Kim,Matthew J. Haney,Yuling Zhao,Vivek Mahajan,I.M. Deygen,Natalia L. Klyachko,Natalia L. Klyachko,Eli Inskoe,Aleksandr Piroyan,Marina Sokolsky,Onyi Okolie,Shawn Hingtgen,Alexander V. Kabanov,Alexander V. Kabanov,Elena V. Batrakova +14 more
TL;DR: A exosome-based system to carry formulation of PTX was developed and showed efficacy in the treatment of multi-drug resistant cancer cells and this novel system may be further developed to carry other chemotherapeutic agents in the future.
Journal ArticleDOI
Mesenchymal Stem Cell-derived Extracellular Vesicles: Toward Cell-free Therapeutic Applications
TL;DR: Current knowledge related to the potential use of MSC-derived EVs in various diseases is reviewed and the promising future for EVs as an alternative, cell-free therapy is discussed.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
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A simple method of estimating fifty per cent endpoints
Lowell J. Reed,H. Muench +1 more
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Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TL;DR: It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).