Robustness of gut microbiota of healthy adults in response to probiotic intervention revealed by high-throughput pyrosequencing
Seok Won Kim,Wataru Suda,Sangwan Kim,Kenshiro Oshima,Shinji Fukuda,Shinji Fukuda,Hiroshi Ohno,Hidetoshi Morita,Masahira Hattori +8 more
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Investigating effects of probiotics on human intestinal microbiota using 454 pyrosequencing of bacterial 16S ribosomal RNA genes with an improved quantitative accuracy suggests robustness of the gut microbiota composition in healthy adults on probiotic administration.Abstract:
Probiotics are live microorganisms that potentially confer beneficial outcomes to host by modulating gut microbiota in the intestine. The aim of this study was to comprehensively investigate effects of probiotics on human intestinal microbiota using 454 pyrosequencing of bacterial 16S ribosomal RNA genes with an improved quantitative accuracy for evaluation of the bacterial composition. We obtained 158 faecal samples from 18 healthy adult Japanese who were subjected to intervention with 6 commercially available probiotics containing either Bifidobacterium or Lactobacillus strains. We then analysed and compared bacterial composition of the faecal samples collected before, during, and after probiotic intervention by Operational taxonomic units (OTUs) and UniFrac distances. The results showed no significant changes in the overall structure of gut microbiota in the samples with and without probiotic administration regardless of groups and types of the probiotics used. We noticed that 32 OTUs (2.7% of all analysed OTUs) assigned to the indigenous species showed a significant increase or decrease of � 10-fold or a quantity difference in >150 reads on probiotic administration. Such OTUs were found to be individual specific and tend to be unevenly distributed in the subjects. These data, thus, suggest robustness of the gut microbiota composition in healthy adults on probiotic administration.read more
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Multiple nutritional and gut microbial factors associated with allergic rhinitis: the Hitachi Health Study
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TL;DR: In this article, the nutritional and gut microbial factors associated with the risk of allergic rhinitis (AR) were examined using the brief-type self-administered diet history questionnaire.
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Book ChapterDOI
Intestinal Microbiota Modulation for Type 1 and Type 2 Diabetes Prevention
TL;DR: The use of probiotics in the treatment of different metabolic disorders especially diabetes has spectacularly improved with special note on the beneficial role of gut microbial community that resides inside us as discussed by the authors .
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A Kawamura,Masaaki Sugita +1 more
TL;DR: In this paper , the effect of omega-3 unsaturated fatty acids via perilla oil intake on gut microbiota and constipation was investigated in a non-randomized placebo-controlled pilot study.
References
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TL;DR: Viewing the microbiota from an ecological perspective could provide insight into how to promote health by targeting this microbial community in clinical treatments.
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