Robustness of gut microbiota of healthy adults in response to probiotic intervention revealed by high-throughput pyrosequencing
Seok Won Kim,Wataru Suda,Sangwan Kim,Kenshiro Oshima,Shinji Fukuda,Shinji Fukuda,Hiroshi Ohno,Hidetoshi Morita,Masahira Hattori +8 more
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Investigating effects of probiotics on human intestinal microbiota using 454 pyrosequencing of bacterial 16S ribosomal RNA genes with an improved quantitative accuracy suggests robustness of the gut microbiota composition in healthy adults on probiotic administration.Abstract:
Probiotics are live microorganisms that potentially confer beneficial outcomes to host by modulating gut microbiota in the intestine. The aim of this study was to comprehensively investigate effects of probiotics on human intestinal microbiota using 454 pyrosequencing of bacterial 16S ribosomal RNA genes with an improved quantitative accuracy for evaluation of the bacterial composition. We obtained 158 faecal samples from 18 healthy adult Japanese who were subjected to intervention with 6 commercially available probiotics containing either Bifidobacterium or Lactobacillus strains. We then analysed and compared bacterial composition of the faecal samples collected before, during, and after probiotic intervention by Operational taxonomic units (OTUs) and UniFrac distances. The results showed no significant changes in the overall structure of gut microbiota in the samples with and without probiotic administration regardless of groups and types of the probiotics used. We noticed that 32 OTUs (2.7% of all analysed OTUs) assigned to the indigenous species showed a significant increase or decrease of � 10-fold or a quantity difference in >150 reads on probiotic administration. Such OTUs were found to be individual specific and tend to be unevenly distributed in the subjects. These data, thus, suggest robustness of the gut microbiota composition in healthy adults on probiotic administration.read more
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Effects of Probiotics and Synbiotics on Obesity, Insulin Resistance Syndrome, Type 2 Diabetes and Non-Alcoholic Fatty Liver Disease: A Review of Human Clinical Trials
María José Sáez-Lara,Cándido Robles-Sánchez,Francisco Javier Ruiz-Ojeda,Julio Plaza-Díaz,Angel Gil +4 more
TL;DR: The oral intake of probiotics and synbiotics as co-adjuvants for the prevention and treatment of obesity, IRS, type 2 diabetes, T2D and NAFLD is partially supported by the data shown, however, further studies are required to understand the precise mechanism of how Probiotics and Synbiotics affect these metabolic disorders.
Journal ArticleDOI
Impact of Diet on Human Intestinal Microbiota and Health
Anne Salonen,Willem M. de Vos +1 more
TL;DR: Current insights on dietary influence on the human intestinal microbiota based on recent high-throughput molecular studies and interconnections with health are reviewed, focusing especially on the emerging data that identify the amount and type of dietary fat as significant modulators of the colonic microbiota and its metabolic output.
Journal ArticleDOI
Dysbiosis and compositional alterations with aging in the gut microbiota of patients with heart failure
Takehiro Kamo,Hiroshi Akazawa,Wataru Suda,Wataru Suda,Akiko Saga-Kamo,Yu Shimizu,Hiroki Yagi,Qing Liu,Seitaro Nomura,Atsuhiko T. Naito,Norifumi Takeda,Mutsuo Harada,Haruhiro Toko,Hidetoshi Kumagai,Yuichi Ikeda,Eiki Takimoto,Jun-ichi Suzuki,Kenya Honda,Hidetoshi Morita,Masahira Hattori,Masahira Hattori,Issei Komuro +21 more
TL;DR: It is suggested that patients with HF harbor significantly altered gut microbiota, which varies further according to age, and new concept of heart-gut axis has a great potential for breakthroughs in the development of novel diagnostic and therapeutic approach for HF.
Journal ArticleDOI
Modulation of Fecal Clostridiales Bacteria and Butyrate by Probiotic Intervention with Lactobacillus paracasei DG Varies among Healthy Adults
Chiara Ferrario,Valentina Taverniti,Christian Milani,Walter Fiore,Monica Laureati,Ivano De Noni,M. Stuknyte,Bessem Chouaia,Patrizia Riso,Simone Guglielmetti +9 more
TL;DR: The intake of L. paracasei DG increased the Blautia:Coprococcus ratio, which, according to the literature, can potentially confer a health benefit on the host, which seems to strictly depend on the initial characteristics of the intestinal microbial ecosystem.
Journal ArticleDOI
The gut microbiota of Colombians differs from that of Americans, Europeans and Asians
TL;DR: In this article, the authors used pyrosequencing of the 16S ribosomal DNA (rDNA) of Colombian adults to compare the results obtained in Americans, Europeans, Japanese and South Koreans, and tested the generality of previous observations concerning changes in Firmicutes and Bacteroidetes with increasing body mass index (BMI).
References
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Catherine A. Lozupone,Jesse Stombaugh,Jeffrey I. Gordon,Janet K. Jansson,Rob Knight,Rob Knight +5 more
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