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Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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CDK12 regulates DNA repair genes by suppressing intronic polyadenylation
TL;DR: The kinaseCDK12 globally suppresses intronic polyadenylation events in mouse embryonic stem cells, enabling the production of full-length gene products and clarifies the function of CDK12 and underscores its potential both as a chemotherapeutic target and as a tumour biomarker.
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Comparison of siRNA-induced off-target RNA and protein effects.
TL;DR: Downregulation of ARGONAUTE 2 (AGO2), the only AGO family protein known to catalyze canonical siRNA-mediated cleavage, did not significantly affect the degree of mRNA knockdown observed for one of the stably expressed reporters after transfection of an imperfectly complementary siRNA.
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Characterization of a highly variable eutherian microRNA gene.
TL;DR: Evidence that a spliced, capped, and polyadenylated primary transcript spans this entire Early Embryonic microRNA Cluster (EEmiRC) is presented and suggests that EEmiRC is a recently acquired rapidly evolving gene important for eutherian development.
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Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP.
TL;DR: Affinity selection experiments revealed that spliceosomes lacking U1 snRNA formed in the U1SnRNP-depleted reactions reconstituted with SR proteins, and high concentrations of SR proteins facilitate the assembly of precursor messenger RNA into aspliceosome in the absence of interactions with U1snRNP.
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Divergent transcription: a driving force for new gene origination?
Xuebing Wu,Phillip A. Sharp +1 more
TL;DR: The mammalian genome is extensively transcribed, a large fraction of which is divergent transcription from promoters and enhancers that is tightly coupled with active gene transcription.