Phillip A. Sharp

TL;DR: In this article, photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq) was performed to identify endogenous miRNA binding sites in mouse embryonic stem cells.

TL;DR: Analysis of Tat trans-activation in vitro will provide new mechanistic insights into this process and allow a more detailed study of the relationship between Tat protein structure and function.

TL;DR: This chapter focuses on three classes of nuclear DNA-dependent RNA polymerases that have been identified in eukaryotic cells, finding that short runoff transcripts have a higher optimum DNA concentration than longer runoff transcripts.

TL;DR: In this paper, the authors investigated if sustained siRNA-mediated silencing of human immunodeficiency virus type 1 (HIV-1) is possible in terminally differentiated macrophages, which constitute an important reservoir of HIV in vivo.

TL;DR: It is demonstrated that Nramp2 is expressed in the apical membrane of the human intestinal epithelial cell line, Caco 2 TC7, and is associated with functional iron transport in these cells with a substrate preference for iron over other divalent cations, suggesting that the expression of the Nramps2 transporter in human enterocytes may play an important role in intestinal iron absorption.