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Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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Journal ArticleDOI
Lariat RNA's as Intermediates and Products in the Splicing of Messenger RNA precursors
Richard A. Padgett,Maria M. Konarska,Maria M. Konarska,Paula J. Grabowski,Steven F. Hardy,Phillip A. Sharp +5 more
TL;DR: The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site, which has been characterized for an adenovirus 2 major late transcript.
Journal ArticleDOI
Pol II phosphorylation regulates a switch between transcriptional and splicing condensates
Yang Eric Guo,John C. Manteiga,Jonathan E. Henninger,Benjamin R. Sabari,Alessandra Dall’Agnese,Nancy M. Hannett,Jan-Hendrik Spille,Jan-Hendrik Spille,Lena K. Afeyan,Alicia V. Zamudio,Krishna Shrinivas,Brian J. Abraham,Brian J. Abraham,Ann Boija,Tim-Michael Decker,Jenna K. Rimel,Charli B. Fant,Tong Ihn Lee,Ibrahim I Cisse,Phillip A. Sharp,Dylan J. Taatjes,Richard A. Young +21 more
TL;DR: In this paper, the authors investigated whether the phosphorylation of the C-terminal domain of the RNA polymerase II (PolII) C-interaction subunit regulates the incorporation of Pol II into phase-separated condensates that are associated with transcription initiation and splicing.
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Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME
TL;DR: From the order of the fragments formed by EcoRI and Hpa I on the adenovirus 2 map, it is concluded that these cell lines contain only the segment of viral DNA that stretches from the left-hand end to a point about 14% along the viral genome, which must be coded by any viral function expressed in transformed cells.
Emerging Roles for Natural MicroRNA Sponges
TL;DR: Questions are raised about the potential ability of thousands of non-coding transcripts to interact with miRNAs and influence the expression of miRNA target genes and some criteria for screening candidate sponge RNAs are considered.
Journal ArticleDOI
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs
TL;DR: Kinetic experiments suggest that complex A is converted with time to a larger, slower migrating complex B, and an endogenous large complex containing U2 snRNP could be detected in nuclear extracts.