P
Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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Journal ArticleDOI
Interactions between RNA polymerase II, factors, and template leading to accurate transcription.
TL;DR: A reconstituted transcription system, consisting of purified RNA polymerase II and three essential HeLa cell chromatographic fractions, is used to study events leading to transcription from the adenovirus major late promoter.
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Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene.
TL;DR: In vivo competition experiments demonstrated that this factor plays a direct role in H-2Kb expression in mouse fibroblasts and the binding site for this factor is TGGGGATTCCCCA, a sequence of perfect dyad symmetry.
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SV40 DNA transfection of cells in suspension: analysis of the efficiency of transcription and translation of T-antigen
Gilbert Chu,Phillip A. Sharp +1 more
TL;DR: A modification of the Graham and Van der Eb (1974) DNA-calcium phosphate coprecipitation technique is shown to routinely transfect 15% of CV-1 cells with SV40 DNA.
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microRNAs: A Safeguard against Turmoil?
TL;DR: It is suggested that microRNAs (miRNAs) are instrumental in a variety of stress responses in addition to their more recognized role in development.
Journal ArticleDOI
T antigen repression of SV40 early transcription from two promoters
TL;DR: D2T, a T antigen analog, specifically represses transcription in vitro from both initiation sites, but at different concentrations, whereas D2T to site I suppresses initiation from the site downstream of the TATA box.