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Phillip A. Sharp

Researcher at Massachusetts Institute of Technology

Publications -  618
Citations -  125567

Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.

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Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins.

TL;DR: A phage called lambdacol100 has been used to introduce the colicin E1 plasmid replicon into Escherichia coli previously treated with chloramphenicol to block protein synthesis as mentioned in this paper.
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Transcription of Simian Virus 40 II. Hybridization of RNA Extracted from Different Lines of Transformed Cells to the Separated Strands of Simian Virus 40 DNA

TL;DR: The amount of simian virus 40 (SV40) DNA present in various SV40-transformed mouse cell lines and "revertants" isolated from them was determined and there was no alteration in the pattern of the stable viral RNA species present in three concanavalin A- selected revertants, whereas in a fluorodeoxyuridine-selected revertant there appeared to be less viral-specific RNA present in the cells.
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Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells

TL;DR: Using human intestinal Caco‐2 TC7 cells, it is shown that iron uptake and DMT1 protein in the plasma membrane were significantly decreased by exposure to high iron for 24 h, in a concentration‐dependent manner, whereas whole cell D MT1 protein abundance was unaltered, which suggests that part of the response to highIron involved redistribution of DMT 1 between the cytosol and cell membrane.
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Association of U2, U4, U5, and U6 small nuclear ribonucleoproteins in a spliceosome-type complex in absence of precursor RNA.

TL;DR: Formation of this U2-U4-U5-U6 (U2/4/5/6) complex, the pseudospliceosome, suggests that the basic structure of the splicingosome is specified by snRNP-snRNP interactions.
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Transcription of Simian Virus 40. III. Mapping of “Early” and “Late” Species of RNA

TL;DR: To determine the orientation of transcription of the E and L strands of DNA from simian virus 40 (SV40), linear DNA prepared by cleavage of superhelical viral DNA by endonuclease R.R(1) from Escherichia coli was used as a primer and RNA extracted from lytically infected cells was hybridized to asymmetric SV40 complementary RNA.