P
Phillip A. Sharp
Researcher at Massachusetts Institute of Technology
Publications - 618
Citations - 125567
Phillip A. Sharp is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: RNA & Gene. The author has an hindex of 172, co-authored 614 publications receiving 117126 citations. Previous affiliations of Phillip A. Sharp include McGovern Institute for Brain Research & Medical Research Council.
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Journal ArticleDOI
Characterizing Polyadenylated uaRNAs Suggests a Potential Role for Pabpn1
Journal ArticleDOI
Characterizing CDK12 and CDK13 Activity in Transcription and mRNA Biogenesis in Mouse Embryonic Stem Cells
Sara J. Dubbury,Phillip A. Sharp +1 more
TL;DR: The RNA Polymerase II (RNAPII) C-terminal domain (CTD) heptapeptide repeat (YSPTSPS) is dynamically phosphorylated during transcription.
Modulator of Mesenchymal Stem Cell Differentiation
Jeong Ho Hong,Eun Sook Hwang,Michael T. McManus,Adam Amsterdam,Yu Tian,Ralitsa Kalmukova,Elisabetta Mueller,Thomas L. Benjamin,Bruce M. Spiegelman,Phillip A. Sharp,Nancy Hopkins,Michael B. Yaffe +11 more
TL;DR: It is reported that a 14-3-3–binding protein, TAZ (transcriptional coactivator with PDZ-binding motif), coactivates Runx2- dependent gene transcription while repressing PPARg-dependent gene transcription, indicating that TAZ functions as a molecular rheostat that modulates MSC differentiation.
Posted ContentDOI
Directin vivomapping of functional suppressors in glioblastoma genome
Ryan D. Chow,Christopher D. Guzman,Guangchuan Wang,Florian I. Schmidt,Mark W. Youngblood,Lupeng Ye,Youssef Errami,Matthew B. Dong,Michael A. Q. Martinez,Sensen Zhang,Paul A. Renauer,Kaya Bilguvar,Murat Gunel,Phillip A. Sharp,Fan Zhang,Randall Jeffrey Platt,Sidi Chen +16 more
TL;DR: This study developed an adeno-associated virus (AAV) mediated autochthonous CRISPR screen and directly mapped functional suppressors in the GBM genome and provides a systematic functional landscape of GBM suppressors directly in vivo, opening new paths for high-throughput molecular mapping and cancer phenotyping.
Posted ContentDOI
Pooled AAV-CRISPR Screen with Targeted Amplicon Sequencing
Guangchuan Wang,Ryan D. Chow,Lupeng Ye,Christopher D. Guzman,Xiaoyun Dai,Matthew B. Dong,Feng Zhang,Phillip A. Sharp,Randall Jeffrey Platt,Sidi Chen +9 more
TL;DR: A new technological approach, Pooled AAV-CRISPR Screen with Targeted Amplicon Sequencing (PASTAS), is developed and demonstrated its application for directly mapping functional cancer driver variants in the mouse liver in an autochthonous manner.