Kyushu University

About: Kyushu University is a education organization based out in Fukuoka, Japan. It is known for research contribution in the topics: Population & Catalysis. The organization has 68284 authors who have published 135190 publications receiving 3055928 citations. The organization is also known as: Kyūshū Daigaku.

Expression of P-glycoprotein in human placenta : relation to genetic polymorphism of the multidrug resistance (MDR)-1 gene

Abstract: To evaluate whether mutations in the human multidrug resistance (MDR)-1 gene correlate with placental P-glycoprotein (PGP) expression, we sequenced the MDR-1 cDNA and measured PGP expression by Western blotting in 100 placentas obtained from Japanese women. Nine single nucleotide polymorphisms (SNPs) were observed with an allelic frequency of 0.005 to 0.420. Of these SNPs, G2677A (allelic frequency = 0.18) and G2677T (0.39) in exon 21 were associated with an amino acid conversion from Ala to Thr and to Ser, respectively. Sixty-one of 65 samples (93.8%), which had a C3435T allele, also had a mutant G2677(A,T) allele, suggesting an association between the two SNPs. Correlations of mutations with expression levels were observed; individuals having the G2677(A,T) and/or T-129C (p < 0.05) allele had less placental PGP. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based genotyping tests were developed for the detection of these SNPs. The PCR, in which genomic DNAs obtained from healthy subjects (n = 48) are used as samples, was successful. The frequency of mutations in placental cDNA was identical with that in genomic DNA. When genotype results were compared between Caucasians and Japanese, ethnic differences in the frequency of polymorphism in the MDR-1 gene were suspected. Although it remains to be determined whether these SNPs influence the pharmacokinetic and dynamic properties of clinically useful drugs that are substrates of PGP, the polymorphism of the MDR-1 gene presented here may provide useful information in in vivo study of these issues.

Formation process of tungsten nanostructure by the exposure to helium plasma under fusion relevant plasma conditions

Abstract: Helium irradiation on tungsten changes the surface morphology dramatically by forming a nanometre-sized fibreform structure which could bring about serious problems for fusion reactors. From the experimental results in liner divertor simulators, it is revealed that the incident ion energy and surface temperature are key parameters for the formation of the structure. It is shown that the tungsten nanostructure is easily formed when the temperature is in the range 1000–2000 K, and the incident ion energy is higher than 20 eV. Furthermore, on the basis of the helium irradiation experiments performed in the divertor simulator NAGDIS-I, the initial formation process of the nanostructure is revealed. It is shown that the nanostructure formation is related to pinholes appearing on the bulk part of the material, and then, the rough structure develops to a much finer nanostructure. The nanostructure was also observed on the molybdenum surface that was exposed to the helium plasma. It increases interest in the possibility that nanostructure formation by helium irradiation is a common phenomenon that occurs on various metals.

Resolution of Resistivity Tomography Inferred from Numerical SIMULATION1

Abstract: Some factors affecting the resolution and accuracy of resistivity tomography are examined using numerical simulation. The inversion method used is based on smoothness-constrained least-squares and finite-element methods. An appropriate block discretization is obtained by dividing the target region into square blocks of size equal to half the minimum electrode spacing. While the effect of the damping factor on the resolution is significant, the resolution is not very sensitive to Gaussian noise as long as the damping factor is properly chosen, according to the noise level. The issue of choosing an optimum electrode array should be considered at the planning stage of a survey. When the instrumental accuracy is high, the dipole-dipole array is more suitable for resolving complex structures than the pole-pole array. The pole-dipole array gives somewhat less resolution than the dipole-dipole array but yields greater signal strength; thus, the pole-dipole array may be a good compromise between resolution and signal strength. The effect of an inhomogeneity located outside the target region may be very small if block discretization is done so as to represent the resistivity variations in both the target and outside regions.

Enhanced insulin secretion and improved glucose tolerance in mice lacking CD26

Abstract: A subset of prolyl oligopeptidases, including dipeptidyl-peptidase IV (DPP IV or CD26, EC 3.4.14.5), specifically cleave off N-terminal dipeptides from substrates having proline or alanine in amino acid position 2. This enzyme activity has been implicated in the regulation of the biological activity of multiple hormones and chemokines, including the insulinotropic peptides glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP). Targeted inactivation of the CD26 gene yielded healthy mice that have normal blood glucose levels in the fasted state, but reduced glycemic excursion after a glucose challenge. Levels of glucose-stimulated circulating insulin and the intact insulinotropic form of GLP-1 are increased in CD26−/− mice. A pharmacological inhibitor of DPP IV enzymatic activity improved glucose tolerance in wild-type, but not in CD26−/−, mice. This inhibitor also improved glucose tolerance in GLP-1 receptor−/− mice, indicating that CD26 contributes to blood glucose regulation by controlling the activity of GLP-1 as well as additional substrates. These data reveal a critical role for CD26 in physiological glucose homeostasis, and establish it as a potential target for therapy in type II diabetes.