Nanjing Tech University

About: Nanjing Tech University is a education organization based out in Nanjing, China. It is known for research contribution in the topics: Catalysis & Membrane. The organization has 21827 authors who have published 21794 publications receiving 364050 citations. The organization is also known as: Nangongda & Nánjīng Gōngyè Dàxúe.

Rational Design of Nanocarriers for Intracellular Protein Delivery.

Abstract: Protein/antibody therapeutics have exhibited the advantages of high specificity and activity even at an extremely low concentration compared to small molecule drugs. However, they are accompanied by unfavorable physicochemical properties such as fragile tertiary structure, large molecular size, and poor penetration of the membrane, and thus the clinical use of protein drugs is hindered by inefficient delivery of proteins into the host cells. To overcome the challenges associated with protein therapeutics and enhance their biopharmaceutical applications, various protein-loaded nanocarriers with desired functions, such as lipid nanocapsules, polymeric nanoparticles, inorganic nanoparticles, and peptides, are developed. In this review, the different strategies for intracellular delivery of proteins are comprehensively summarized. Their designed routes, mechanisms of action, and potential therapeutics in live cells or in vivo are discussed in detail. Furthermore, the perspective on the new generation of delivery systems toward the emerging area of protein-based therapeutics is presented as well.

Fabrication of gold nanoparticles on bilayer graphene for glucose electrochemical biosensing

Abstract: The hydrophilic and carboxyl group functionalized graphene–gold nanoparticles (AuNPs) hybrid has been synthesized in situ. AuNPs can be scattered well on the graphene bilayer, and the loading amount of AuNPs can be controlled. Glucose oxidase (GOD) was successfully bound to the surface of the hybrid through a condensation reaction between terminal amino groups on the lysine residues of GOD and carboxyl groups on the AuNPs. The hybrid provided a suitable microenvironment for GOD to retain its biological activity. The direct and reversible electron transfer process between GOD and the hybrid electrode was realized without any supporting film or electron mediator. A novel model of the glucose biosensor based on the hybrid electrode was fabricated. Blood sugar concentrations measured in human serum samples by the glucose biosensor were in good agreement with the values provided by the Nanjing University hospital, and the average relative standard deviation was 3.2% for six successive measurements. Three constructed biosensors showed good stability, and all of them retained 80% of their initial signals after they were stored at 4 °C for four months. It is promising that the model of the glucose biosensor can be used as an effective candidate for the detection of blood sugar concentration in clinical diagnoses.

3D Printed Flexible Strain Sensors: From Printing to Devices and Signals

Abstract: The revolutionary and pioneering advancements of flexible electronics provide the boundless potential to become one of the leading trends in the exploitation of wearable devices and electronic skin. Working as substantial intermediates for the collection of external mechanical signals, flexible strain sensors that get intensive attention are regarded as indispensable components in flexible integrated electronic systems. Compared with conventional preparation methods including complicated lithography and transfer printing, 3D printing technology is utilized to manufacture various flexible strain sensors owing to the low processing cost, superior fabrication accuracy, and satisfactory production efficiency. Herein, up-to-date flexible strain sensors fabricated via 3D printing are highlighted, focusing on different printing methods based on photocuring and materials extrusion, including Digital Light Processing (DLP), fused deposition modeling (FDM), and direct ink writing (DIW). Sensing mechanisms of 3D printed strain sensors are also discussed. Furthermore, the existing bottlenecks and future prospects are provided for further progressing research.