Institution
University of Bordeaux
Education•Bordeaux, France•
About: University of Bordeaux is a education organization based out in Bordeaux, France. It is known for research contribution in the topics: Population & Laser. The organization has 28811 authors who have published 55536 publications receiving 1619635 citations. The organization is also known as: UB.
Topics: Population, Laser, Context (language use), Raman spectroscopy, Medicine
Papers published on a yearly basis
Papers
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TL;DR: It is demonstrated that the single nickase and not the nuclease approach is preferable, not only for modeling disease but also and more importantly for the safe management of future CRISPR-Cas9-mediated gene therapies.
Abstract: CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nucleaseinduced double-strand break DNA (DSB) at the UROS locus to model and correct congenital erythropoietic porphyria. We demonstrate that homology-directed repair is rare compared with NHEJ pathway leading to on-target indels and causing unwanted dysfunctional protein. Moreover, we describe unexpected chromosomal truncations resulting from only one Cas9 nuclease-induced DSB in cell lines and primary cells by a p53-dependent mechanism. Altogether, these side effects may limit the promising perspectives of the CRISPR-Cas9 nuclease system for disease modeling and gene therapy. We show that the single nickase approach could be safer since it prevents on-and off-target indels and chromosomal truncations. These results demonstrate that the single nickase and not the nuclease approach is preferable, not only for modeling disease but also and more importantly for the safe management of future CRISPR-Cas9-mediated gene therapies.
249 citations
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TL;DR: This primer will review the different approaches used to track single molecules in live neurons, compare them to bulk measurements, and discuss the different concepts that have emerged from their application to synaptic biology.
249 citations
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TL;DR: In this paper, the authors show that the Lazaret Bay (Toulon Harbour) is highly contaminated by polyaromatic hydrocarbons (PAHs) and that pyrolytic origin is the main source of these compounds.
248 citations
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TL;DR: It was observed that the maximum metal uptake was reached in less than 2 h and a decline in solution proton concentration (pH increase) and release of calcium, magnesium, potassium and sodium from the cocoa shells was demonstrated.
248 citations
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TL;DR: Michal Kucera, Antoni Rosell-Mele, Ralph Schneider, Claire Waelbroeck, Mara Weinelt Department of Geology, Royal Holloway University of London, Egham TW20 0EX, UK ICREA and Institute of Environmental Science and Technology, Universitat Autonoma de Barcelona, 08193 Bellaterra, Catalonia, Spain Departement de Geologie et Oceanographie, UMR5805-EPOC, CNRS/Universite de Bordeaux1, 33405 Talence Cedex, France
247 citations
Authors
Showing all 28995 results
Name | H-index | Papers | Citations |
---|---|---|---|
Nicholas G. Martin | 192 | 1770 | 161952 |
George F. Koob | 171 | 935 | 112521 |
Daniel J. Jacob | 162 | 656 | 76530 |
Arthur W. Toga | 159 | 1184 | 109343 |
James M. Tour | 143 | 859 | 91364 |
Floyd E. Bloom | 139 | 616 | 72641 |
Herbert Y. Meltzer | 137 | 1148 | 81371 |
Jean-Marie Tarascon | 136 | 853 | 137673 |
Stanley Nattel | 132 | 778 | 65700 |
Michel Haïssaguerre | 117 | 757 | 62284 |
Liquan Chen | 111 | 689 | 44229 |
Marion Leboyer | 110 | 773 | 50767 |
Jean-François Dartigues | 106 | 631 | 46682 |
Alexa S. Beiser | 106 | 366 | 47457 |
Robert Dantzer | 105 | 497 | 46554 |