Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti.
Takakazu Kaneko,Yasukazu Nakamura,Shusei Sato,Erika Asamizu,Tomohiko Kato,Shigemi Sasamoto,Akiko Watanabe,Kumi Idesawa,Atsuko Ishikawa,Kumiko Kawashima,Takaharu Kimura,Yoshie Kishida,Chiaki Kiyokawa,Mitsuyo Kohara,Midori Matsumoto,Ai Matsuno,Yoko Mochizuki,Shinobu Nakayama,Naomi Nakazaki,Sayaka Shimpo,Masako Sugimoto,Chie Takeuchi,Manabu Yamada,Satoshi Tabata +23 more
TLDR
The complete nucleotide sequence of the genome of a symbiotic bacterium Mesorhizobium loti strain MAFF303099 was determined and suggested that the symbiotic island as well as the plasmids originated and were transmitted from other genetic systems.Abstract:
The complete nucleotide sequence of the genome of a symbiotic bacterium Mesorhizobium loti strain MAFF303099 was determined. The genome of M. loti consisted of a single chromosome (7,036, 071 bp) and two plasmids, designated as pMLa (351,911 bp) and pMLb (208,315 bp). The chromosome comprises 6752 potential protein-coding genes, two sets of rRNA genes and 50 tRNA genes representing 47 tRNA species. Fifty-four percent of the potential protein genes showed sequence similarity to genes of known function, 21% to hypothetical genes, and the remaining 25% had no apparent similarity to reported genes. A 611-kb DNA segment, a highly probable candidate of a symbiotic island, was identified, and 30 genes for nitrogen fixation and 24 genes for nodulation were assigned in this region. Codon usage analysis suggested that the symbiotic island as well as the plasmids originated and were transmitted from other genetic systems. The genomes of two plasmids, pMLa and pMLb, contained 320 and 209 potential protein-coding genes, respectively, for a variety of biological functions. These include genes for the ABC-transporter system, phosphate assimilation, two-component system, DNA replication and conjugation, but only one gene for nodulation was identified.read more
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Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)
Stephen D. Bentley,Keith F. Chater,Ana Cerdeño-Tárraga,Gregory L. Challis,Gregory L. Challis,Nicholas R. Thomson,Keith D. James,David Harris,Michael A. Quail,H. M. Kieser,D. Harper,Alex Bateman,Steve D.M. Brown,Govind Chandra,Carton W. Chen,Mark O. Collins,Ann Cronin,Andrew G. Fraser,Arlette Goble,J. Hidalgo,T. Hornsby,S. Howarth,Chih-Hung Huang,Tobias Kieser,L. Larke,Lee Murphy,Karen Oliver,Susan O'Neil,Ester Rabbinowitsch,Marie-Adèle Rajandream,Kim Rutherford,Simon Rutter,Kathy Seeger,David L. Saunders,Sarah Sharp,R. Squares,S. Squares,K. Taylor,T. Warren,Andreas Wietzorrek,John Woodward,Bart Barrell,Julian Parkhill,David A. Hopwood +43 more
TL;DR: The 8,667,507 base pair linear chromosome of Streptomyces coelicolor is reported, containing the largest number of genes so far discovered in a bacterium.
Journal ArticleDOI
The genome sequence of Schizosaccharomyces pombe
Valerie Wood,R. Gwilliam,Marie-Adèle Rajandream,M. Lyne,Rachel Lyne,A. Stewart,J. Sgouros,N. Peat,Jacqueline Hayles,Stephen Baker,D. Basham,Sharen Bowman,Karen Brooks,D. Brown,Steve D.M. Brown,Tracey Chillingworth,Carol Churcher,Mark O. Collins,R. Connor,Ann Cronin,P. Davis,Theresa Feltwell,Andrew G. Fraser,S. Gentles,Arlette Goble,N. Hamlin,David Harris,J. Hidalgo,Geoffrey M. Hodgson,S. Holroyd,T. Hornsby,S. Howarth,Elizabeth J. Huckle,Sarah E. Hunt,Kay Jagels,Kylie R. James,L. Jones,Matthew Jones,S. Leather,S. McDonald,J. McLean,P. Mooney,Sharon Moule,Karen Mungall,Lee Murphy,D. Niblett,C. Odell,Karen Oliver,Susan O'Neil,D. Pearson,Michael A. Quail,Ester Rabbinowitsch,Kim Rutherford,Simon Rutter,David L. Saunders,Kathy Seeger,Sarah Sharp,Jason Skelton,Mark Simmonds,R. Squares,S. Squares,K. Stevens,K. Taylor,Ruth Taylor,Adrian Tivey,S. Walsh,T. Warren,S. Whitehead,John Woodward,Guido Volckaert,Rita Aert,Johan Robben,B. Grymonprez,I. Weltjens,E. Vanstreels,Michael A. Rieger,M. Schafer,S. Muller-Auer,C. Gabel,M. Fuchs,C. Fritzc,E. Holzer,D. Moestl,H. Hilbert,K. Borzym,I. Langer,Alfred Beck,Hans Lehrach,Richard Reinhardt,Thomas M. Pohl,P. Eger,Wolfgang Zimmermann,H. Wedler,R. Wambutt,Bénédicte Purnelle,André Goffeau,Edouard Cadieu,Stéphane Dréano,Stéphanie Gloux,Valerie Lelaure,Stéphanie Mottier,Francis Galibert,Stephen J. Aves,Z. Xiang,Cherryl Hunt,Karen Moore,S. M. Hurst,M. Lucas,M. Rochet,Claude Gaillardin,Victor A. Tallada,Victor A. Tallada,Andrés Garzón,Andrés Garzón,G. Thode,Rafael R. Daga,Rafael R. Daga,L. Cruzado,Juan Jimenez,Juan Jimenez,Miguel del Nogal Sánchez,F. del Rey,J. Benito,Angel Domínguez,José L. Revuelta,Sergio Moreno,John Armstrong,Susan L. Forsburg,L. Cerrutti,Todd M. Lowe,W. R. McCombie,Ian T. Paulsen,Judith A. Potashkin,G. V. Shpakovski,David W. Ussery,Bart Barrell,Paul Nurse +136 more
TL;DR: The genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote, is sequenced and highly conserved genes important for eukARYotic cell organization including those required for the cytoskeleton, compartmentation, cell-cycle control, proteolysis, protein phosphorylation and RNA splicing are identified.
Journal ArticleDOI
Bacteria with ACC deaminase can promote plant growth and help to feed the world
TL;DR: It is argued that the ability of plant growth-promoting bacteria that produce 1-aminocyclopropane-1-carboxylate (ACC) deaminase to lower plant ethylene levels, often a result of various stresses, is a key component in the efficacious functioning of these bacteria.
Journal ArticleDOI
Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis
Haruo Ikeda,Jun Ishikawa,Akiharu Hanamoto,Mayumi Shinose,Hisashi Kikuchi,Tadayoshi Shiba,Yoshiyuki Sakaki,Masahira Hattori,Satoshi Omura +8 more
TL;DR: The complete nucleotide sequence of the linear chromosome of Streptomyces avermitilis is determined and it is revealed that an internal 6.5-Mb region in the S. avermitILis genome was highly conserved with respect to gene order and content, and contained all known essential genes but showed perfectly asymmetric structure at the oriC center.
Journal ArticleDOI
The composite genome of the legume symbiont Sinorhizobium meliloti.
Francis Galibert,Turlough M. Finan,Sharon R. Long,Sharon R. Long,Alfred Pühler,Pia Abola,Frédéric Ampe,Frédérique Barloy-Hubler,Melanie J. Barnett,Anke Becker,P. Boistard,G. Bothe,Marc Boutry,Leah Bowser,Jens Buhrmester,Edouard Cadieu,Delphine Capela,Delphine Capela,Patrick S. G. Chain,Alison Cowie,Ronald W. Davis,Stéphane Dréano,Nancy A. Federspiel,Robert F. Fisher,Stéphanie Gloux,Thérèse Godrie,André Goffeau,Brian Golding,Jérôme Gouzy,Mani Gurjal,Ismael Hernández-Lucas,Andrea Hong,Lucas Huizar,Richard W. Hyman,Ted Jones,Daniel Kahn,Michael L. Kahn,Sue Kalman,David H. Keating,David H. Keating,Ernö Kiss,Caridad Komp,Valerie Lelaure,David Masuy,Curtis J. Palm,Melicent C. Peck,Thomas M. Pohl,Daniel Portetelle,Bénédicte Purnelle,Uwe Ramsperger,Raymond Surzycki,Patricia Thebault,Micheline Vandenbol,Frank-J. Vorhölter,Stefan Weidner,Derek H. Wells,Kim Wong,Kuo-Chen Yeh,Kuo-Chen Yeh,Jacques Batut +59 more
TL;DR: The annotated DNA sequence of the α-proteobacteriumSinorhizobium meliloti, the symbiont of alfalfa, is presented, indicating that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution.
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