Differences in the localization and morphology of chromosomes in the human nucleus
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TLDR
It is demonstrated that the distribution of genomic sequences between chromosomes has implications for nuclear structure and the findings are discussed in relation to a model of the human nucleus that is functionally compartmentalized.Abstract:
Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19. Human chromosome 19 is shown to adopt a more internal position in the nucleus than chromosome 18 and to be more extensively associated with the nuclear matrix. The more peripheral localization of chromosome 18 is established early in the cell cycle and is maintained thereafter. We show that the preferential localization of chromosomes 18 and 19 in the nucleus is reflected in the orientation of translocation chromosomes in the nucleus. Lastly, we show that the inhibition of transcription can have gross, but reversible, effects on chromosome architecture. Our data demonstrate that the distribution of genomic sequences between chromosomes has implications for nuclear structure and we discuss our findings in relation to a model of the human nucleus that is functionally compartmentalized.read more
Citations
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Artificially introduced aneuploid chromosomes assume a conserved position in colon cancer cells.
Kundan Sengupta,Madhvi B. Upender,Linda Barenboim-Stapleton,Quang Tri Nguyen,Stephen Wincovitch,Susan H. Garfield,Michael J. Difilippantonio,Thomas Ried +7 more
TL;DR: Using 3D-FISH and confocal laser scanning microscopy, it is shown that Chromosomes 7, 18, or 19 introduced via microcell-mediated chromosome transfer into the parental diploid colon cancer cell line DLD-1 maintain their conserved position in the interphase nucleus.
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Gene density and chromosome territory shape.
Nitasha Sehgal,Andrew Fritz,Kristen Morris,Irianna Torres,Zihe Chen,Jinhui Xu,Ronald Berezney +6 more
TL;DR: A strong inverse correlation between the degree of regular CT shape and gene density for those CT that are most gene-rich (19, 17, 11) and gene-poor (18, 13, Y) is demonstrated, while CT more intermediate in gene density showed a strong negative correlation with shape regularity, but not with ellipticity.
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Into the Fourth Dimension: Dysregulation of Genome Architecture in Aging and Alzheimer's Disease.
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Association of the telomere-telomere-binding protein complex of hypotrichous ciliates with the nuclear matrix and dissociation during replication
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Nucleolus association of chromosomal domains is largely maintained in cellular senescence despite massive nuclear reorganisation
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References
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The inactive X chromosome in female mammals is distinguished by a lack of histone H4 acetylation, a cytogenetic marker for gene expression
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A Physical Map of 30,000 Human Genes
Panos Deloukas,Gregory D. Schuler,G. Gyapay,E. M. Beasley,Carol Soderlund,P. Rodriguez-Tomé,L. Hui,Tara C. Matise,K. B. McKusick,Jacques S. Beckmann,S. Bentolila,M.-T. Bihoreau,B. B. Birren,J. Browne,Adam Butler,A. B. Castle,N. Chiannilkulchai,C. Clee,P. J. R. Day,Anindya Dehejia,T. Dibling,N. Drouot,S. Duprat,C. Fizames,Sidney W. Fox,S. Gelling,L. Green,Paul Harrison,R. Hocking,E. Holloway,Sarah E. Hunt,S. Keil,Philip Lijnzaad,C. Louis-Dit-Sully,Jianpeng Ma,A. Mendis,J.H. Miller,J. Morissette,D. Muselet,H. C. Nusbaum,A. Peck,Steve Rozen,D. Simon,Donna K. Slonim,R. Staples,L. D. Stein,E. A. Stewart,Marc A. Suchard,T. Thangarajah,N. Vega-Czarny,Caleb Webber,Xufeng S. Wu,James R. Hudson,Charles Auffray,N. Nomura,James M. Sikela,Mihael H. Polymeropoulos,M. R. James,Eric S. Lander,Thomas J. Hudson,Richard M. Myers,D. R. Cox,Jean Weissenbach,Mark S. Boguski,D. R. Bentley +64 more
TL;DR: A map of 30,181 human gene-based markers was assembled and integrated with the current genetic map by radiation hybrid mapping, which contains nearly twice as many genes as the previous release and is twofold to threefold more accurate than the previous version.
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