Differences in the localization and morphology of chromosomes in the human nucleus
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TLDR
It is demonstrated that the distribution of genomic sequences between chromosomes has implications for nuclear structure and the findings are discussed in relation to a model of the human nucleus that is functionally compartmentalized.Abstract:
Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19. Human chromosome 19 is shown to adopt a more internal position in the nucleus than chromosome 18 and to be more extensively associated with the nuclear matrix. The more peripheral localization of chromosome 18 is established early in the cell cycle and is maintained thereafter. We show that the preferential localization of chromosomes 18 and 19 in the nucleus is reflected in the orientation of translocation chromosomes in the nucleus. Lastly, we show that the inhibition of transcription can have gross, but reversible, effects on chromosome architecture. Our data demonstrate that the distribution of genomic sequences between chromosomes has implications for nuclear structure and we discuss our findings in relation to a model of the human nucleus that is functionally compartmentalized.read more
Citations
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Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development.
Steven T. Kosak,Jane A. Skok,Kay L. Medina,Roy Riblet,Michelle M. Le Beau,Amanda G. Fisher,Harinder Singh +6 more
TL;DR: It is suggested that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igκ loci during lymphocyte development.
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Genome architecture: domain organization of interphase chromosomes
TL;DR: Together with microscopy and computational modeling, the results begin to yield a more coherent picture that integrates linear and three-dimensional views of chromosome organization in relation to gene regulation and other nuclear functions.
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A t(1;19)(q10;p10) Mediates the Combined Deletions of 1p and 19q and Predicts a Better Prognosis of Patients with Oligodendroglioma
Robert B. Jenkins,Hilary E. Blair,Karla V. Ballman,Caterina Giannini,Robert M. Arusell,Mark E. Law,Heather C. Flynn,Sandra M. Passe,Sara J. Felten,Paul D. Brown,Edward G. Shaw,Jan C. Buckner +11 more
TL;DR: The results strongly suggest that a t(1;19)(q10;p10) mediates the combined 1p/19q deletion in human gliomas and the 1;19 translocation is associated with superior OS and progression-free survival in low-grade glioma patients.
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The Hierarchy of the 3D Genome
Johan H. Gibcus,Job Dekker +1 more
TL;DR: The experimental and theoretical data on this hierarchy of structures and a key role for the recently discovered topologically associating domains are reviewed and proposed.
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The spatial organization of human chromosomes within the nuclei of normal and emerin-mutant cells
Shelagh Boyle,Susan Gilchrist,Joanna M. Bridger,Nicola L. Mahy,Juliet A. Ellis,Wendy A. Bickmore +5 more
TL;DR: The intranuclear organization of chromosomes is not altered in cells that lack the integral nuclear membrane protein emerin, from an individual with X-linked Emery--Dreifuss muscular dystrophy, which suggests that emerin is not necessary for localizing chromosomes at the nuclear periphery and that the muscular Dystrophy phenotype in such individuals is not due to grossly altered nuclear organization of chromatin.
References
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TL;DR: Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells and a family of attachment sites related by hybridization to those of the hsp70 genes was discovered.
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TL;DR: It is shown that transcriptionally inactive but not transcriptionally active genes associate with Ikaros-heterochromatin foci, which support a model of organization of the nucleus in which repressed genes are selectively recruited into centromeric domains.
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The inactive X chromosome in female mammals is distinguished by a lack of histone H4 acetylation, a cytogenetic marker for gene expression
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Journal ArticleDOI
A Physical Map of 30,000 Human Genes
Panos Deloukas,Gregory D. Schuler,G. Gyapay,E. M. Beasley,Carol Soderlund,P. Rodriguez-Tomé,L. Hui,Tara C. Matise,K. B. McKusick,Jacques S. Beckmann,S. Bentolila,M.-T. Bihoreau,B. B. Birren,J. Browne,Adam Butler,A. B. Castle,N. Chiannilkulchai,C. Clee,P. J. R. Day,Anindya Dehejia,T. Dibling,N. Drouot,S. Duprat,C. Fizames,Sidney W. Fox,S. Gelling,L. Green,Paul Harrison,R. Hocking,E. Holloway,Sarah E. Hunt,S. Keil,Philip Lijnzaad,C. Louis-Dit-Sully,Jianpeng Ma,A. Mendis,J.H. Miller,J. Morissette,D. Muselet,H. C. Nusbaum,A. Peck,Steve Rozen,D. Simon,Donna K. Slonim,R. Staples,L. D. Stein,E. A. Stewart,Marc A. Suchard,T. Thangarajah,N. Vega-Czarny,Caleb Webber,Xufeng S. Wu,James R. Hudson,Charles Auffray,N. Nomura,James M. Sikela,Mihael H. Polymeropoulos,M. R. James,Eric S. Lander,Thomas J. Hudson,Richard M. Myers,D. R. Cox,Jean Weissenbach,Mark S. Boguski,D. R. Bentley +64 more
TL;DR: A map of 30,181 human gene-based markers was assembled and integrated with the current genetic map by radiation hybrid mapping, which contains nearly twice as many genes as the previous release and is twofold to threefold more accurate than the previous version.
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