Differences in the localization and morphology of chromosomes in the human nucleus
Reads0
Chats0
TLDR
It is demonstrated that the distribution of genomic sequences between chromosomes has implications for nuclear structure and the findings are discussed in relation to a model of the human nucleus that is functionally compartmentalized.Abstract:
Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19. Human chromosome 19 is shown to adopt a more internal position in the nucleus than chromosome 18 and to be more extensively associated with the nuclear matrix. The more peripheral localization of chromosome 18 is established early in the cell cycle and is maintained thereafter. We show that the preferential localization of chromosomes 18 and 19 in the nucleus is reflected in the orientation of translocation chromosomes in the nucleus. Lastly, we show that the inhibition of transcription can have gross, but reversible, effects on chromosome architecture. Our data demonstrate that the distribution of genomic sequences between chromosomes has implications for nuclear structure and we discuss our findings in relation to a model of the human nucleus that is functionally compartmentalized.read more
Citations
More filters
Journal ArticleDOI
When 3D genome technology meets viral infection, including SARS‐CoV‐2
TL;DR: The research progress on the three‐dimensional interaction between virus and host genome and the impact of virus integration into the host genome on gene transcription regulation is summarized to provide new insights into chromatin interaction and viral gene transcriptionregulation, laying the foundation for the treatment of infectious diseases.
Journal ArticleDOI
Specific features in linear and spatial organizations of pericentromeric heterochromatin regions in polytene chromosomes of the closely related species Drosophila virilis and D. kanekoi (Diptera: Drosophilidae)
TL;DR: It is suggested that chromosome rearrangements play an important role in the redistribution of heterochromatin DNA sequences in the genome, representing a speciation mechanism, which, in general, could also affect the chromosome orientation in the 3D nuclear space.
Book ChapterDOI
Functional Consequences of Nuclear and Nucleolar Architecture
Eva Bártová,Lenka Stixová +1 more
TL;DR: It is increasingly clear that changes in global genome organization and chromatin dynamics occur in parallel with functional changes in the genome.
Journal ArticleDOI
Inverted rod nuclei see the light.
TL;DR: This nuclear architecture is inverted in rod cells of nocturnal mammals, demonstrating a unique functional nuclear genome reorganization specifically adapted for light transmission.
References
More filters
Journal ArticleDOI
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold
TL;DR: Data are presented for sequence-specific chromatin-loop organization in histone-depleted nuclei from Drosophila melanogaster Kc cells and a family of attachment sites related by hybridization to those of the hsp70 genes was discovered.
Journal ArticleDOI
Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells.
Dean A. Jackson,Ana Pombo +1 more
TL;DR: It is proposed that the coordinated replication of related groups of replicons, that form stable replicon clusters, contributes to the efficient activation and propagation of S phase in mammalian cells.
Journal ArticleDOI
Association of Transcriptionally Silent Genes with Ikaros Complexes at Centromeric Heterochromatin
Karen E. Brown,Simon Guest,Stephen T. Smale,Kyungmin Hahm,Matthias Merkenschlager,Amanda G. Fisher +5 more
TL;DR: It is shown that transcriptionally inactive but not transcriptionally active genes associate with Ikaros-heterochromatin foci, which support a model of organization of the nucleus in which repressed genes are selectively recruited into centromeric domains.
Journal ArticleDOI
The inactive X chromosome in female mammals is distinguished by a lack of histone H4 acetylation, a cytogenetic marker for gene expression
Peter Jeppesen,Bryan M. Turner +1 more
TL;DR: In this paper, immunolabeled human and mouse metaphase chromosomes with antibodies specific for the acetylated isoforms of histone H4 were labeled in regions corresponding to conventional R bands (regions enriched in coding DNA), except for a single chromosome in female cells.
Journal ArticleDOI
A Physical Map of 30,000 Human Genes
Panos Deloukas,Gregory D. Schuler,G. Gyapay,E. M. Beasley,Carol Soderlund,P. Rodriguez-Tomé,L. Hui,Tara C. Matise,K. B. McKusick,Jacques S. Beckmann,S. Bentolila,M.-T. Bihoreau,B. B. Birren,J. Browne,Adam Butler,A. B. Castle,N. Chiannilkulchai,C. Clee,P. J. R. Day,Anindya Dehejia,T. Dibling,N. Drouot,S. Duprat,C. Fizames,Sidney W. Fox,S. Gelling,L. Green,Paul Harrison,R. Hocking,E. Holloway,Sarah E. Hunt,S. Keil,Philip Lijnzaad,C. Louis-Dit-Sully,Jianpeng Ma,A. Mendis,J.H. Miller,J. Morissette,D. Muselet,H. C. Nusbaum,A. Peck,Steve Rozen,D. Simon,Donna K. Slonim,R. Staples,L. D. Stein,E. A. Stewart,Marc A. Suchard,T. Thangarajah,N. Vega-Czarny,Caleb Webber,Xufeng S. Wu,James R. Hudson,Charles Auffray,N. Nomura,James M. Sikela,Mihael H. Polymeropoulos,M. R. James,Eric S. Lander,Thomas J. Hudson,Richard M. Myers,D. R. Cox,Jean Weissenbach,Mark S. Boguski,D. R. Bentley +64 more
TL;DR: A map of 30,181 human gene-based markers was assembled and integrated with the current genetic map by radiation hybrid mapping, which contains nearly twice as many genes as the previous release and is twofold to threefold more accurate than the previous version.
Related Papers (5)
Chromosome territories, nuclear architecture and gene regulation in mammalian cells.
Comprehensive mapping of long-range interactions reveals folding principles of the human genome.
Erez Lieberman Aiden,Nynke L. van Berkum,Louise Williams,Maxim Imakaev,Tobias Ragoczy,Tobias Ragoczy,Agnes Telling,Agnes Telling,Ido Amit,Bryan R. Lajoie,Peter J. Sabo,Michael O. Dorschner,Richard Sandstrom,Bradley E. Bernstein,Bradley E. Bernstein,Michaël Bender,Mark Groudine,Mark Groudine,Andreas Gnirke,John A. Stamatoyannopoulos,Leonid A. Mirny,Eric S. Lander,Eric S. Lander,Job Dekker +23 more