Differentially expressed microRNAs in experimental cerebral malaria and their involvement in endocytosis, adherens junctions, FoxO and TGF-β signalling pathways.
Aarón Martin-Alonso,Amy Cohen,María Antonieta Quispe-Ricalde,Pilar Foronda,Agustín Benito,Pedro Berzosa,Basilio Valladares,Georges E. Grau +7 more
TLDR
The data implies that, at least in the mouse model, miRNA may play a regulatory role in CM pathogenesis, and suggests that these miRNA, through their regulation of downstream targets, may be vitally involved in the neurological syndrome.Abstract:
Cerebral malaria (CM) is the most severe manifestation of infection with Plasmodium, however its pathogenesis is still not completely understood. microRNA (miRNA) have been an area of focus in infectious disease research, due to their ability to affect normal biological processes, and have been shown to play roles in various viral, bacterial and parasitic infections, including malaria. The expression of miRNA was studied following infection of CBA mice with either Plasmodium berghei ANKA (causing CM), or Plasmodium yoelii (causing severe but non-cerebral malaria (NCM)). Using microarray analysis, miRNA expression was compared in the brains of non-infected (NI), NCM and CM mice. Six miRNA were significantly dysregulated between NCM and CM mice, and four of these, miR-19a-3p, miR-19b-3p, miR-142-3p and miR-223-3p, were further validated by qPCR assays. These miRNA are significantly involved in several pathways relevant to CM, including the TGF-β and endocytosis pathways. Dysregulation of these miRNA during CM specifically compared with NCM suggests that these miRNA, through their regulation of downstream targets, may be vitally involved in the neurological syndrome. Our data implies that, at least in the mouse model, miRNA may play a regulatory role in CM pathogenesis.read more
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MicroRNAs: Biological Regulators in Pathogen-Host Interactions.
TL;DR: The biological aspects of microRNAs are reviewed, focusing on their roles as regulators of gene expression during pathogen–host interactions and their implications in the immune response against Leishmania, Trypanosoma, Toxoplasma, and Plasmodium infectious diseases.
Journal ArticleDOI
LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway.
TL;DR: Exosomes obtained from MIF-pretreated MSCs have a protective effect on cardiomyocytes and lncRNA-NEAT1 functions as an anti-apoptotic molecule via competitive endogenous RNA activity towards miR-142-3p.
Journal ArticleDOI
Noncoding RNAs in Parasite-Vector-Host Interactions.
TL;DR: An overview of the involvement of ncRNAs in the parasite-vector-host triad and their effect on host homeostasis based on recent advances and accumulating knowledge about the role of endogenous vertebrate noncoding RNAs in vertebrate host physiology is provided.
Journal ArticleDOI
The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients
Nutpakal Ketprasit,Iris Simone Cheng,Fiona Deutsch,Nham Tran,Mallika Imwong,Valery Combes,Duangdao Palasuwan +6 more
TL;DR: Bioinformatic analysis revealed that EV specific miRNAs might regulate genes involved in the malaria pathway including the adherens junction and the transforming growth factor-β pathways and could potentially be used as disease biomarkers as determined by AUC.
Journal ArticleDOI
Non-coding RNAs: Emerging from the discovery to therapeutic applications
TL;DR: In this paper, a review of the different types of non-coding RNAs (ncRNAs) is presented, by describing their biogenesis, functions, and applications, and a perspective on the main challenges and innovative approaches for the future and broad therapeutic application of RNA is also presented.
References
More filters
Journal ArticleDOI
Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method
TL;DR: The 2-Delta Delta C(T) method as mentioned in this paper was proposed to analyze the relative changes in gene expression from real-time quantitative PCR experiments, and it has been shown to be useful in the analysis of realtime, quantitative PCR data.
Journal ArticleDOI
MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
Journal ArticleDOI
KEGG: Kyoto Encyclopedia of Genes and Genomes
Minoru Kanehisa,Susumu Goto +1 more
TL;DR: The Kyoto Encyclopedia of Genes and Genomes (KEGG) as discussed by the authors is a knowledge base for systematic analysis of gene functions in terms of the networks of genes and molecules.
Journal ArticleDOI
Regulated portals of entry into the cell
Sean D. Conner,Sandra L. Schmid +1 more
TL;DR: ‘Endocytosis’ encompasses several diverse mechanisms by which cells internalize macromolecules and particles into transport vesicles derived from the plasma membrane and must be viewed in a broader context than simple vesicular trafficking.
Journal ArticleDOI
Antiviral Immunity Directed by Small RNAs
Shou-Wei Ding,Olivier Voinnet +1 more
TL;DR: The proteins required for viRNA production as well as several key downstream components of the antiviral immunity pathway have been identified in plants, flies, and worms, illuminating an ongoing molecular arms race that likely impacts the evolution of both viral and host genomes.
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