Efficient Transposition of the piggyBac (PB) Transposon in Mammalian Cells and Mice
TLDR
It is shown that piggyBac elements carrying multiple genes can efficiently transpose in human and mouse cell lines and also in mice, providing a first and critical step toward a highly efficient transposon system for a variety of genetic manipulations in mice and other vertebrates.About:
This article is published in Cell.The article was published on 2005-08-12 and is currently open access. It has received 875 citations till now. The article focuses on the topics: PiggyBac Transposon System & Sleeping Beauty transposon system.read more
Citations
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A global double‐fluorescent Cre reporter mouse
Mandar Deepak Muzumdar,Bosiljka Tasic,Bosiljka Tasic,Kazunari Miyamichi,Kazunari Miyamichi,Ling Li,Ling Li,Liqun Luo,Liqun Luo +8 more
TL;DR: The mT/mG mouse as mentioned in this paper is a double-fluorescent Cre reporter mouse that expresses membrane-targeted tandem dimer tomato (mT) prior to Cre-mediated excision and membranetargeted green fluorescent protein (mG) after excision.
Journal ArticleDOI
Critical nodes in signalling pathways: Insights into insulin action
TL;DR: The concept of 'critical nodes' is used to define the important junctions in these pathways and illustrate their unique role using insulin signalling as a model system.
Journal ArticleDOI
piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
Knut Woltjen,Iacovos P. Michael,Iacovos P. Michael,Paria Mohseni,Paria Mohseni,Ridham Desai,Ridham Desai,Maria Mileikovsky,Riikka H. Hämäläinen,Rebecca Cowling,Wei Wang,Pentao Liu,Marina Gertsenstein,Keisuke Kaji,Hoon Ki Sung,Andras Nagy,Andras Nagy +16 more
TL;DR: It is shown that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery, and the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines is demonstrated.
Journal ArticleDOI
Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
TL;DR: P piggyBac transposon–based reprogramming may be used to generate therapeutically applicable iPSCs and could be identified by negative selection.
Journal ArticleDOI
A hyperactive piggyBac transposase for mammalian applications.
TL;DR: A unique hyperactive piggyBac transposase is generated with 17-fold and ninefold increases in excision and integration, respectively, and its applicability is shown by demonstrating an increased efficiency of generation of transgene-free mouse induced pluripotent stem cells.
References
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TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
Journal ArticleDOI
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TL;DR: The results of an international collaboration to produce a high-quality draft sequence of the mouse genome are reported and an initial comparative analysis of the Mouse and human genomes is presented, describing some of the insights that can be gleaned from the two sequences.
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Manipulating the mouse embryo: A laboratory manual
TL;DR: Here are recorded the tech- niques for preparing, inserting and analysing DNA sequences, for retroviral infection of mice, for production and use of EC and EK cells as vehicles for engineered sequences and for nuclear transplantation - all against a background of the basic procedures required for pro- ducing and handling the em- bryos.
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