Journal ArticleDOI
Emerging evidence for functional peptides encoded by short open reading frames
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TLDR
A review of short open reading frames (sORFs) in the proteome can be found in this paper, where the authors consider potential roles for sORF-encoded peptides.Abstract:
Short open reading frames (sORFs) are a common feature of all genomes, but their coding potential has mostly been disregarded, partly because of the difficulty in determining whether these sequences are translated. Recent innovations in computing, proteomics and high-throughput analyses of translation start sites have begun to address this challenge and have identified hundreds of putative coding sORFs. The translation of some of these has been confirmed, although the contribution of their peptide products to cellular functions remains largely unknown. This Review examines this hitherto overlooked component of the proteome and considers potential roles for sORF-encoded peptides.read more
Citations
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A Micropeptide Encoded by a Putative Long Noncoding RNA Regulates Muscle Performance
Douglas M. Anderson,Kelly M. Anderson,Chi-Lun Chang,Catherine A. Makarewich,Benjamin R. Nelson,John R. McAnally,Prasad Kasaragod,John M. Shelton,Jen Liou,Rhonda Bassel-Duby,Eric N. Olson +10 more
TL;DR: Findings identify myoregulin (MLN) as an important regulator of skeletal muscle physiology and highlight the possibility that additional micropeptides are encoded in the many RNAs currently annotated as noncoding.
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The functions and unique features of long intergenic non-coding RNA.
TL;DR: Long intergenic non-coding RNA genes have diverse features that distinguish them from mRNA-encoding genes and exercise functions such as remodelling chromatin and genome architecture, RNA stabilization and transcription regulation, including enhancer-associated activity.
Book ChapterDOI
History, Discovery, and Classification of lncRNAs.
TL;DR: The many discoveries that led to the study of lncRNAs are discussed, from Friedrich Miescher's "nuclein" in 1869 to the elucidation of the human genome and transcriptome in the early 2000s, to focus on the biological relevance during lncRNA evolution and describe their basic features as genes and transcripts.
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A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma
Maolei Zhang,Kun Zhao,Xiaoping Xu,Yibing Yang,Sheng Yan,Sheng Yan,Ping Wei,Hui Liu,Jianbo Xu,Feizhe Xiao,Huangkai Zhou,Xuesong Yang,Nunu Huang,Jinglei Liu,Kejun He,Keping Xie,Gong Zhang,Suyun Huang,Nu Zhang +18 more
TL;DR: A 87-amino-acid peptide encoded by the circular form of the long intergenic non-protein-coding RNA p53-induced transcript (LINC-PINT) is identified that can reduce glioblastoma proliferation via interaction with PAF1 which sequentially inhibits the transcriptional elongation of some oncogenes.
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Long non-coding RNAs as a source of new peptides
TL;DR: It is found that a large fraction of the lncRNAs expressed in cells from six different species is associated with ribosomes, indicating that they play an important role in de novo protein evolution.
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TL;DR: Functional data from multiple, diverse experiments performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE Project are reported, providing convincing evidence that the genome is pervasively transcribed, such that the majority of its bases can be found in primary transcripts.
Journal ArticleDOI
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
TL;DR: 5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs and GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates.
Journal ArticleDOI
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
TL;DR: By analyzing the effects of single base substitutions around the ATG initiator codon in a cloned preproinsulin gene, ACCATGG is identified as the optimal sequence for initiation by eukaryotic ribosomes.