Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
TLDR
A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.Abstract:
Objectives
Identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.read more
Citations
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High genomic diversity of multi-drug resistant wastewater Escherichia coli
Norhan Mahfouz,Serena Caucci,Serena Caucci,Eric Achatz,Torsten Semmler,Sebastian Guenther,Sebastian Guenther,Thomas U. Berendonk,Michael Schroeder +8 more
TL;DR: In this article, the authors analyzed and quantified the genomic diversity of the indicator Escherichia coli in a German wastewater treatment plant and related it to isolates' antibiotic resistance, showing a surprisingly large pan-genome, which mirrors how rich an environment a treatment plant is.
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Colistin Resistance Mediated by mcr-1 in ESBL-Producing, Multidrug Resistant Salmonella Infantis in Broiler Chicken Industry, Italy (2016-2017).
Virginia Carfora,Patricia Alba,Pimlapas Leekitcharoenphon,Daniele Ballarò,Gessica Cordaro,Paola Di Matteo,Valentina Donati,Angela Ianzano,Manuela Iurescia,Fiorentino Stravino,Tania Tagliaferri,Antonio Battisti,Alessia Franco +12 more
TL;DR: This research presents a novel probabilistic approach to estimating the response of the immune system to laser-spot assisted, 3D image analysis of central nervous system injury.
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Horizontal Gene Transfer and Acquired Antibiotic Resistance in Salmonella enterica Serovar Heidelberg following In Vitro Incubation in Broiler Ceca.
Adelumola Oladeinde,Kimberly L. Cook,Steven M. Lakin,Reed Woyda,Zaid Abdo,Torey Looft,Kyler Herrington,Gregory Zock,Jodie Plumblee Lawrence,Jesse C. Thomas,Megan S. Beaudry,Travis C. Glenn +11 more
TL;DR: In this paper, the authors used broiler chicken cecum as a model to investigate antibiotic resistance genes that can be transferred in vitro from cecal flora to Salmonella enterica serovar Heidelberg.
Journal ArticleDOI
Genomic and functional characterisation of IncX3 plasmids encoding blaSHV-12 in Escherichia coli from human and animal origin
Apostolos Liakopoulos,Jeanet van der Goot,Alex Bossers,Jonathan W. Betts,Michael S.M. Brouwer,Arie Kant,Hilde E. Smith,Daniela Ceccarelli,Dik Mevius +8 more
TL;DR: The findings indicate that plasmids of this subgroup are conjugative and highly stable, while they exert no fitness cost on their bacterial host, and might have contributed to the emergence of IncX3 amongst SHV-12-producing E. coli in the Netherlands.
Journal ArticleDOI
Stepwise evolution of Salmonella Typhimurium ST313 causing bloodstream infection in Africa.
Caisey V. Pulford,Blanca M. Perez-Sepulveda,Rocío Canals,Jessica A. Bevington,Rebecca J. Bengtsson,Nicolas Wenner,Ella V. Rodwell,Benjamin Kumwenda,Xiaojun Zhu,Rebecca J. Bennett,George E. Stenhouse,P. Malaka De Silva,Hermione J. Webster,José A. Bengoechea,Amy Dumigan,Alicia Tran-Dien,Reenesh Prakash,Happy C. Banda,Lovemore Alufandika,Mike P. Mautanga,Arthur Bowers-Barnard,Alexandra Beliavskaia,Alexander V. Predeus,Will P. M. Rowe,Alistair C. Darby,Neil Hall,François-Xavier Weill,Melita A. Gordon,Nicholas A. Feasey,Kate S. Baker,Jay C. D. Hinton +30 more
TL;DR: In this paper, a pan-susceptible Salmonella Typhimurium sequence type (ST)313 lineage (ST313 L3) was discovered in Malawi in 2016 and is closely related to ST313 variants that cause gastrointestinal disease in the United Kingdom and Brazil.
References
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Journal ArticleDOI
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria
Mette Voldby Larsen,Salvatore Cosentino,Simon Rasmussen,Carsten Friis,Henrik Hasman,Rasmus L. Marvig,Lars Jelsbak,Thomas Sicheritz-Pontén,David W. Ussery,Frank Møller Aarestrup,Ole Lund +10 more
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Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
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Journal ArticleDOI
Acquired Antibiotic Resistance Genes: An Overview
Angela H.A.M. van Hoek,Dik Mevius,Dik Mevius,Beatriz Guerra,Peter Mullany,Adam P. Roberts,Henk Aarts +6 more
TL;DR: Attention is paid to mobile genetic elements such as plasmids, transposons, and integrons, which are associated with AR genes, and involved in the dispersal of antimicrobial determinants between different bacteria.
Journal ArticleDOI
Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
Lance B. Price,Marc Stegger,Henrik Hasman,Maliha Aziz,Jesper Larsen,Paal Skytt Andersen,Talima Pearson,Andrew E. Waters,Jeffrey T. Foster,James M. Schupp,John D. Gillece,Elizabeth M. Driebe,Cindy M. Liu,Cindy M. Liu,Burkhard Springer,Irena Zdovc,Antonio Battisti,Alessia Franco,Jacek Żmudzki,Stefan Schwarz,Patrick Butaye,Eric Jouy,Constança Pomba,M. Concepción Porrero,Raymond Ruimy,Tara C. Smith,D. Ashley Robinson,J. Scott Weese,Carmen Sofia Arriola,Fangyou Yu,Frédéric Laurent,Paul Keim,Paul Keim,Robert Skov,Frank Møller Aarestrup +34 more
TL;DR: The results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA, which appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance.
Journal ArticleDOI
Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark.
TL;DR: Differences in the occurrence of resistance and tetracycline resistance genes were observed among isolates from the different sources, however, similar resistance patterns and resistant genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs.
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