Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
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TLDR
A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.Abstract:
Objectives
Identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.read more
Citations
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Journal ArticleDOI
Polyphyletic Nature of Salmonella enterica Serotype Derby and Lineage-Specific Host-Association Revealed by Genome-Wide Analysis.
Yann Sévellec,Marie-Léone Vignaud,Sophie A. Granier,Renaud Lailler,Carole Feurer,Simon Le Hello,Michel-Yves Mistou,Sabrina Cadel-Six +7 more
TL;DR: A phylogenetic analysis demonstrates the polyphyletic nature of the Salmonella serotype Derby and provides an opportunity to identify genetic factors associated with host adaptation and markers for the monitoring of these different lineages within the corresponding animal sectors.
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Associations between antimicrobial use and the faecal resistome on broiler farms from nine European countries
Roosmarijn E C Luiken,Liese Van Gompel,Patrick Munk,Steven Sarrazin,Philip Joosten,Alejandro Dorado-García,Rasmus Borup Hansen,Berith Elkær Knudsen,Alex Bossers,Jaap A. Wagenaar,Frank Møller Aarestrup,Jeroen Dewulf,Dik Mevius,Dick Heederik,Lidwien A.M. Smit,Heike Schmitt +15 more
TL;DR: The faecal microbiome in European broilers contains a high diversity of ARGs, even in the absence of current antimicrobial selection pressure, despite this, the relative abundance of genes and the composition of the resistome is positively related to AMU in Europe broiler farms for several antimicrobial classes.
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Antibiotic resistance: bioinformatics-based understanding as a functional strategy for drug design
TL;DR: Understanding the evolution of antibiotic resistance at the molecular level as a functional tool for bioinformatic-based drug design is an important step in the fight against antibiotic resistance.
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Discovery of a novel integron-borne aminoglycoside resistance gene present in clinical pathogens by screening environmental bacterial communities.
Maria-Elisabeth Böhm,Mohammad Razavi,Nachiket P. Marathe,Carl-Fredrik Flach,D. G. Joakim Larsson +4 more
TL;DR: This is the first time a novel resistance gene, present in clinical isolates, has been discovered by exploring the environmental microbiome, and its specificity to garosamine-containing aminoglycosides may reduce the usefulness of the newest semisynthetic am inoglycoside plazomicin, which is designed to avoid common aminglycoside resistance mechanisms.
Journal ArticleDOI
Genetic Contexts of blaNDM-1 in Patients Carrying Multiple NDM-Producing Strains
Alexander M. Wailan,Anna L. Sartor,Hosam M. Zowawi,Hosam M. Zowawi,John D. Perry,David L. Paterson,Hanna E. Sidjabat +6 more
TL;DR: Combining genetic context characterization with other molecular epidemiology methods, the molecular epidemiological links between genetically unrelated bacterial species are established by linking their acquisition of an IncN2 or IncA/C plasmid carrying blaNDM-1 for carbapenem resistance.
References
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Journal ArticleDOI
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria
Mette Voldby Larsen,Salvatore Cosentino,Simon Rasmussen,Carsten Friis,Henrik Hasman,Rasmus L. Marvig,Lars Jelsbak,Thomas Sicheritz-Pontén,David W. Ussery,Frank Møller Aarestrup,Ole Lund +10 more
TL;DR: A Web-based method for MLST of 66 bacterial species based on whole-genome sequencing data that enables investigators to determine the sequence types of their isolates on the basis of WGS data.
Journal ArticleDOI
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
TL;DR: A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.
Journal ArticleDOI
Acquired Antibiotic Resistance Genes: An Overview
Angela H.A.M. van Hoek,Dik Mevius,Dik Mevius,Beatriz Guerra,Peter Mullany,Adam P. Roberts,Henk Aarts +6 more
TL;DR: Attention is paid to mobile genetic elements such as plasmids, transposons, and integrons, which are associated with AR genes, and involved in the dispersal of antimicrobial determinants between different bacteria.
Journal ArticleDOI
Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
Lance B. Price,Marc Stegger,Henrik Hasman,Maliha Aziz,Jesper Larsen,Paal Skytt Andersen,Talima Pearson,Andrew E. Waters,Jeffrey T. Foster,James M. Schupp,John D. Gillece,Elizabeth M. Driebe,Cindy M. Liu,Cindy M. Liu,Burkhard Springer,Irena Zdovc,Antonio Battisti,Alessia Franco,Jacek Żmudzki,Stefan Schwarz,Patrick Butaye,Eric Jouy,Constança Pomba,M. Concepción Porrero,Raymond Ruimy,Tara C. Smith,D. Ashley Robinson,J. Scott Weese,Carmen Sofia Arriola,Fangyou Yu,Frédéric Laurent,Paul Keim,Paul Keim,Robert Skov,Frank Møller Aarestrup +34 more
TL;DR: The results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA, which appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance.
Journal ArticleDOI
Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark.
TL;DR: Differences in the occurrence of resistance and tetracycline resistance genes were observed among isolates from the different sources, however, similar resistance patterns and resistant genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs.
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