Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
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TLDR
A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.Abstract:
Objectives
Identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.read more
Citations
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Methicillin-resistant staphylococci amongst veterinary personnel, personnel-owned pets, patients and the hospital environment of two small animal veterinary hospitals.
TL;DR: There was limited MRS transmission between veterinary personnel, their pets, patients or the veterinary environment, and MRSA isolates were resistant to significantly more antimicrobial classes than MRSA.
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Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms.
Andrea Garretto,Taylor Miller-Ensminger,Adriana Ene,Zubia Merchant,Aashaka C. Shah,Athina Gerodias,Anthony Biancofiori,Stacey Canchola,Stephanie Canchola,Emanuel Castillo,Tasnim Chowdhury,Nikita Gandhi,Sarah Hamilton,Kyla Hatton,Syed Hyder,Koty Krull,Demetrios Lagios,Thinh Lam,Kennedy Mitchell,Christine Mortensen,Amber Murphy,Joseph Richburg,Meghan Rokas,Suzanne Ryclik,Pauline Sulit,Thomas Szwajnos,Manuel Widuch,Jessica Willis,Mary Woloszyn,Bridget Brassil,Genevieve Johnson,Rita Mormando,Laura Maskeri,Mary Batrich,Nicole Stark,Jason W. Shapiro,Cesar Montelongo Hernandez,Swarnali Banerjee,Alan J. Wolfe,Catherine Putonti +39 more
TL;DR: It is hypothesized that UTI symptoms associated with detection of E. coli are more likely the result of urobiome composition, rather than the presence of the virus itself, as previously found.
Journal ArticleDOI
Characterization of a Linezolid- and Vancomycin-Resistant Streptococcus suis Isolate That Harbors optrA and vanG Operons.
TL;DR: This is the first report of LVR isolate, which is mediated by acquired resistance genes optrA and vanG operons in Gram-positive bacteria, and the potential risks of disseminating of optr a andVanG from S. suis to other Streptococcus spp are worrisome and routine surveillance should be strengthened.
Journal ArticleDOI
A Systematic Approach to Identify and Characterize the Effectiveness and Safety of Novel Probiotic Strains to Control Foodborne Pathogens.
Diana I. Ayala,Peter W. Cook,Peter W. Cook,Jorge G. Franco,Marie Bugarel,Kameswara Rao Kottapalli,Guy H. Loneragan,Mindy M. Brashears,Kendra K. Nightingale +8 more
TL;DR: It is suggested that the selected panel of LAB probiotic strains can be used as biocontrol cultures to inhibit and/or reduce the growth of L. monocytogenes, Salmonella, and E. coli O157:H7 in different matrices, and environments.
Journal ArticleDOI
Identification of novel tetracycline resistance gene tet(X14) and its co-occurrence with tet(X2) in a tigecycline-resistant and colistin-resistant Empedobacter stercoris.
Yingying Cheng,Yingying Cheng,Yong Chen,Yong Chen,Yang Liu,Yang Liu,Yuqi Guo,Yuqi Guo,Yanzi Zhou,Tingting Xiao,Shuntian Zhang,Hao Xu,Yunbo Chen,Tongling Shan,Yonghong Xiao,Kai Zhou,Kai Zhou +16 more
TL;DR: The expanded members of Tet(X) family warrants the potential large-scale dissemination and the necessity of continuous surveillance for tet(X)-mediated tigecycline resistance.
References
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Journal ArticleDOI
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria
Mette Voldby Larsen,Salvatore Cosentino,Simon Rasmussen,Carsten Friis,Henrik Hasman,Rasmus L. Marvig,Lars Jelsbak,Thomas Sicheritz-Pontén,David W. Ussery,Frank Møller Aarestrup,Ole Lund +10 more
TL;DR: A Web-based method for MLST of 66 bacterial species based on whole-genome sequencing data that enables investigators to determine the sequence types of their isolates on the basis of WGS data.
Journal ArticleDOI
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
TL;DR: A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.
Journal ArticleDOI
Acquired Antibiotic Resistance Genes: An Overview
Angela H.A.M. van Hoek,Dik Mevius,Dik Mevius,Beatriz Guerra,Peter Mullany,Adam P. Roberts,Henk Aarts +6 more
TL;DR: Attention is paid to mobile genetic elements such as plasmids, transposons, and integrons, which are associated with AR genes, and involved in the dispersal of antimicrobial determinants between different bacteria.
Journal ArticleDOI
Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
Lance B. Price,Marc Stegger,Henrik Hasman,Maliha Aziz,Jesper Larsen,Paal Skytt Andersen,Talima Pearson,Andrew E. Waters,Jeffrey T. Foster,James M. Schupp,John D. Gillece,Elizabeth M. Driebe,Cindy M. Liu,Cindy M. Liu,Burkhard Springer,Irena Zdovc,Antonio Battisti,Alessia Franco,Jacek Żmudzki,Stefan Schwarz,Patrick Butaye,Eric Jouy,Constança Pomba,M. Concepción Porrero,Raymond Ruimy,Tara C. Smith,D. Ashley Robinson,J. Scott Weese,Carmen Sofia Arriola,Fangyou Yu,Frédéric Laurent,Paul Keim,Paul Keim,Robert Skov,Frank Møller Aarestrup +34 more
TL;DR: The results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA, which appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance.
Journal ArticleDOI
Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark.
TL;DR: Differences in the occurrence of resistance and tetracycline resistance genes were observed among isolates from the different sources, however, similar resistance patterns and resistant genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs.
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