Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
TLDR
A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.Abstract:
Objectives
Identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.read more
Citations
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Journal ArticleDOI
Whole Genome Sequencing of Extended Spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae Isolated from Hospitalized Patients in KwaZulu-Natal, South Africa
TL;DR: This study showed that both district and tertiary hospital in uMgungundlovu District were reservoirs for several resistance determinants and highlighted the necessity to efficiently and routinely screen patients, particularly those receiving extensive antibiotic treatment and long-term hospitalization stay.
Journal ArticleDOI
Exploring the Potential of CRISPR-Cas9 Under Challenging Conditions: Facing High-Copy Plasmids and Counteracting Beta-Lactam Resistance in Clinical Strains of Enterobacteriaceae.
Thaysa Leite Tagliaferri,Thaysa Leite Tagliaferri,Natália Rocha Guimarães,Marcella de Paula Martins Pereira,Liza Figueiredo Felicori Vilela,Hans-Peter Horz,Simone Gonçalves dos Santos,Tiago Antônio de Oliveira Mendes +7 more
TL;DR: The data show that targeting drug resistance genes is encouraging even when facing high-copy plasmids, and in clinical isolates, the simultaneous interference with multiple genes mediating overlapping drug resistance might be the clue for successful phenotype reversal.
Journal ArticleDOI
Emergence of new variants of antibiotic resistance genomic islands among multidrug-resistant Salmonella enterica in poultry.
Emiliano Cohen,Maya Davidovich,Assaf Rokney,Lea Valinsky,Galia Rahav,Galia Rahav,Ohad Gal-Mor,Ohad Gal-Mor +7 more
TL;DR: The role of the poultry production as a pool of specific epidemic MDR strains and autonomous genetic elements, which confer resistance to heavy metals and medically relevant antibiotics, are emphasized, likely to disseminate to humans via the food chain and fuel the increasing global antibiotic resistance crisis.
Journal ArticleDOI
Dissecting the molecular evolution of fluoroquinolone-resistant Shigella sonnei
Christine J. Boinett,Duy Pham Thanh,Claire Jenkins,François-Xavier Weill,Benjamin P Howden,Mary Valcanis,Niall De Lappe,Martin Cormican,Sonam Wangchuk,Ladaporn Bodhidatta,Carl J. Mason,To Nguyen Thi Nguyen,Tuyen Ha Thanh,Vinh Phat Voong,Vu Thuy Duong,Phu H. Nguyen,Paul Turner,Paul Turner,Ryan R. Wick,Pieter-Jan Ceyssens,Guy E. Thwaites,Kathryn E. Holt,Kathryn E. Holt,Nicholas R. Thomson,Nicholas R. Thomson,Maia A. Rabaa,Stephen Baker,Stephen Baker +27 more
TL;DR: Results indicate that sequential accumulation of defining mutations led to the emergence of the fluoroquinolone-resistant S. sonnei population around 2007 in South Asia, and suggests that the clone became dominant through enhanced adaptation to oxidative stress.
Journal ArticleDOI
Biofilm formation, virulence and antimicrobial resistance of different Campylobacter jejuni isolates from a poultry slaughterhouse.
TL;DR: Characters of factors such as biofilm formation, virulence and antimicrobial resistance in environmental isolates and the possible link between the phenotypic and genetic characterization using whole genome sequencing (WGS) suggest that multiple mechanisms may exist that allow these organisms to persist and ultimately cause disease in humans.
References
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Journal ArticleDOI
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria
Mette Voldby Larsen,Salvatore Cosentino,Simon Rasmussen,Carsten Friis,Henrik Hasman,Rasmus L. Marvig,Lars Jelsbak,Thomas Sicheritz-Pontén,David W. Ussery,Frank Møller Aarestrup,Ole Lund +10 more
TL;DR: A Web-based method for MLST of 66 bacterial species based on whole-genome sequencing data that enables investigators to determine the sequence types of their isolates on the basis of WGS data.
Journal ArticleDOI
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
TL;DR: A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.
Journal ArticleDOI
Acquired Antibiotic Resistance Genes: An Overview
Angela H.A.M. van Hoek,Dik Mevius,Dik Mevius,Beatriz Guerra,Peter Mullany,Adam P. Roberts,Henk Aarts +6 more
TL;DR: Attention is paid to mobile genetic elements such as plasmids, transposons, and integrons, which are associated with AR genes, and involved in the dispersal of antimicrobial determinants between different bacteria.
Journal ArticleDOI
Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
Lance B. Price,Marc Stegger,Henrik Hasman,Maliha Aziz,Jesper Larsen,Paal Skytt Andersen,Talima Pearson,Andrew E. Waters,Jeffrey T. Foster,James M. Schupp,John D. Gillece,Elizabeth M. Driebe,Cindy M. Liu,Cindy M. Liu,Burkhard Springer,Irena Zdovc,Antonio Battisti,Alessia Franco,Jacek Żmudzki,Stefan Schwarz,Patrick Butaye,Eric Jouy,Constança Pomba,M. Concepción Porrero,Raymond Ruimy,Tara C. Smith,D. Ashley Robinson,J. Scott Weese,Carmen Sofia Arriola,Fangyou Yu,Frédéric Laurent,Paul Keim,Paul Keim,Robert Skov,Frank Møller Aarestrup +34 more
TL;DR: The results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA, which appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance.
Journal ArticleDOI
Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark.
TL;DR: Differences in the occurrence of resistance and tetracycline resistance genes were observed among isolates from the different sources, however, similar resistance patterns and resistant genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs.
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