Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
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TLDR
A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.Abstract:
Objectives
Identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.read more
Citations
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Journal ArticleDOI
Genomic epidemiology of campylobacter jejuni associated with asymptomatic pediatric infection in the peruvian amazon
Ben Pascoe,Ben Pascoe,Francesca Schiaffino,Francesca Schiaffino,Susan Murray,Susan Murray,Guillaume Méric,Sion C. Bayliss,Matthew D. Hitchings,Evangelos Mourkas,Jessica K. Calland,Rosa Burga,Pablo Peñataro Yori,Keith A. Jolley,Kerry K. Cooper,Craig T. Parker,Maribel Paredes Olortegui,Margaret Kosek,Samuel K. Sheppard,Samuel K. Sheppard,Samuel K. Sheppard +20 more
TL;DR: As in many other countries, poultry-associated strains were likely a major source of human infection but almost half of local disease cases were attributable to genotypes that are rare outside of Peru, suggesting an important role for host factors in the cryptic epidemiology of campylobacteriosis in LMICs.
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Outcome of Different Sequencing and Assembly Approaches on the Detection of Plasmids and Localization of Antimicrobial Resistance Genes in Commensal Escherichia coli.
Katharina Juraschek,Maria Borowiak,Simon H. Tausch,Burkhard Malorny,Annemarie Käsbohrer,Saria Otani,Stefan Schwarz,Diana Meemken,Carlus Deneke,Jens A. Hammerl +9 more
TL;DR: In this paper, different sequencing and assembly strategies were tested for their suitability in AMR monitoring in Escherichia coli in the routines of the German National Reference Laboratory for Antimicrobial Resistances.
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Prevalence, antimicrobial resistance and phylogenetic characterization of Yersinia enterocolitica in retail poultry meat and swine feces in parts of China
Zixin Peng,Mingyuan Zou,Menghan Li,Danru Liu,Wenying Guan,Qiong Hao,Jin Xu,Shuhong Zhang,Huaiqi Jing,Ying Li,Xiang Liu,Dongmin Yu,Shaofei Yan,Wei Wang,Fengqin Li +14 more
TL;DR: Analysis of retail poultry meat samples in China revealed the emergence of drug-resistant pathogenic Y. entrocoliticas in retailed poultry meats in China and indicated that pathogenic isolates were conserved on genetic level.
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Antibiotic-Resistant Enterobacteriaceae in Wastewater of Abattoirs.
Timo Homeier-Bachmann,Stefan E. Heiden,Phillip K. Lübcke,Lisa Bachmann,Jürgen A. Bohnert,Dirk Zimmermann,Katharina Schaufler +6 more
TL;DR: In this paper, the authors analyzed the wastewater from seven slaughterhouses (pig and poultry) for extended-spectrum β-lactamase (ESBL)-carrying and colistin-resistant Enterobacteriaceae.
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Characteristics of Shiga toxin producing- and enteropathogenic Escherichia coli of the emerging serotype O80:H2 isolated from humans and diarrhoeic calves in Belgium.
TL;DR: The human STEC and bovine aEPEC cluster together and have the potential to be as virulent as the French isolates, as shown by their similar gene content.
References
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Journal ArticleDOI
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria
Mette Voldby Larsen,Salvatore Cosentino,Simon Rasmussen,Carsten Friis,Henrik Hasman,Rasmus L. Marvig,Lars Jelsbak,Thomas Sicheritz-Pontén,David W. Ussery,Frank Møller Aarestrup,Ole Lund +10 more
TL;DR: A Web-based method for MLST of 66 bacterial species based on whole-genome sequencing data that enables investigators to determine the sequence types of their isolates on the basis of WGS data.
Journal ArticleDOI
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
TL;DR: A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.
Journal ArticleDOI
Acquired Antibiotic Resistance Genes: An Overview
Angela H.A.M. van Hoek,Dik Mevius,Dik Mevius,Beatriz Guerra,Peter Mullany,Adam P. Roberts,Henk Aarts +6 more
TL;DR: Attention is paid to mobile genetic elements such as plasmids, transposons, and integrons, which are associated with AR genes, and involved in the dispersal of antimicrobial determinants between different bacteria.
Journal ArticleDOI
Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
Lance B. Price,Marc Stegger,Henrik Hasman,Maliha Aziz,Jesper Larsen,Paal Skytt Andersen,Talima Pearson,Andrew E. Waters,Jeffrey T. Foster,James M. Schupp,John D. Gillece,Elizabeth M. Driebe,Cindy M. Liu,Cindy M. Liu,Burkhard Springer,Irena Zdovc,Antonio Battisti,Alessia Franco,Jacek Żmudzki,Stefan Schwarz,Patrick Butaye,Eric Jouy,Constança Pomba,M. Concepción Porrero,Raymond Ruimy,Tara C. Smith,D. Ashley Robinson,J. Scott Weese,Carmen Sofia Arriola,Fangyou Yu,Frédéric Laurent,Paul Keim,Paul Keim,Robert Skov,Frank Møller Aarestrup +34 more
TL;DR: The results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA, which appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance.
Journal ArticleDOI
Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark.
TL;DR: Differences in the occurrence of resistance and tetracycline resistance genes were observed among isolates from the different sources, however, similar resistance patterns and resistant genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs.
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