Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
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TLDR
A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.Abstract:
Objectives
Identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.read more
Citations
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Journal ArticleDOI
Transfer of a bla CTX-M-1-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses.
Per Kristian Knudsen,Per Kristian Knudsen,Karianne Wiger Gammelsrud,Karianne Wiger Gammelsrud,Kristian Alfsnes,Martin Steinbakk,Tore G. Abrahamsen,Tore G. Abrahamsen,Fredrik Müller,Fredrik Müller,Jon Bohlin +10 more
TL;DR: The findings strongly suggest that horizontal transfer of a blaCTX-M-1-carrying plasmid had occurred within the patient´s gut and highlights the value of collecting multiple bacterial colonies from longitudinally collected samples to assess faecal carriage of resistant enterobacteria.
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Genomic data reveal international lineages of critical priority Escherichia coli harbouring wide resistome in Andean condors (Vultur gryphus Linnaeus, 1758)
Danny Fuentes-Castillo,Fernanda Esposito,Brenda Cardoso,Gislaine Taimara Dalazen,Quézia Moura,Bruna Fuga,Herrison Fontana,Louise Cerdeira,Milena Dropa,Jürgen Rottmann,Daniel González-Acuña,José Luiz Catão-Dias,Nilton Lincopan +12 more
TL;DR: The results highlight that Andean Condors have been colonized by critical priority pathogens, becoming potential environmental reservoirs and/or vectors for dissemination of virulent and antimicrobial‐resistant bacteria and/ or their genes, in associated ecosystems and wildlife.
Journal ArticleDOI
Exploring potential of vaginal Lactobacillus isolates from South African women for enhancing treatment for bacterial vaginosis.
Anna-Ursula Happel,Brian Kullin,Hoyam Gamieldien,Nicole Wentzel,Chambrez Z. Zauchenberger,Heather B. Jaspan,Heather B. Jaspan,Smritee Dabee,Smritee Dabee,Shaun L. Barnabas,Shaun L. Barnabas,Shameem Z. Jaumdally,Janan Dietrich,Janan Dietrich,Glenda Gray,Glenda Gray,Linda-Gail Bekker,Linda-Gail Bekker,Rémy Froissart,Jo-Ann S. Passmore,Jo-Ann S. Passmore,Jo-Ann S. Passmore +21 more
TL;DR: In this paper, Lactobacillus strains from South African women were evaluated for their probiotic potential in vitro compared to strains from commercial vaginal products, including growth at varying pHs, ability to lower pH, produce D-/L-lactate and H2O2, influence growth of BV-associated Gardnerella vaginalis and Prevotella bivia, adherence to cervical cells and susceptibility to antibiotics.
Journal ArticleDOI
Biocide-Tolerant Listeria monocytogenes Isolates from German Food Production Plants Do Not Show Cross-Resistance to Clinically Relevant Antibiotics.
Alice Roedel,Ralf Dieckmann,Holger Brendebach,Jens A. Hammerl,Sylvia Kleta,Matthias Noll,S. Al Dahouk,Szilvia Vincze +7 more
TL;DR: The results do not provide evidence for increasing cross-resistance to antibiotics in field isolates, and a high genetic diversity among the investigated isolates including genotypes that are frequently involved in human infections are demonstrated.
Journal ArticleDOI
Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains
Lucie Bardet,Jean-Marc Rolain +1 more
TL;DR: This review aims to summarize the current clinical microbiology diagnosis techniques and their ability to detect all colistin resistance mechanisms and describe new tools specifically developed to assess plasmid-mediated colistsin resistance.
References
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Journal ArticleDOI
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria
Mette Voldby Larsen,Salvatore Cosentino,Simon Rasmussen,Carsten Friis,Henrik Hasman,Rasmus L. Marvig,Lars Jelsbak,Thomas Sicheritz-Pontén,David W. Ussery,Frank Møller Aarestrup,Ole Lund +10 more
TL;DR: A Web-based method for MLST of 66 bacterial species based on whole-genome sequencing data that enables investigators to determine the sequence types of their isolates on the basis of WGS data.
Journal ArticleDOI
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
TL;DR: A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.
Journal ArticleDOI
Acquired Antibiotic Resistance Genes: An Overview
Angela H.A.M. van Hoek,Dik Mevius,Dik Mevius,Beatriz Guerra,Peter Mullany,Adam P. Roberts,Henk Aarts +6 more
TL;DR: Attention is paid to mobile genetic elements such as plasmids, transposons, and integrons, which are associated with AR genes, and involved in the dispersal of antimicrobial determinants between different bacteria.
Journal ArticleDOI
Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
Lance B. Price,Marc Stegger,Henrik Hasman,Maliha Aziz,Jesper Larsen,Paal Skytt Andersen,Talima Pearson,Andrew E. Waters,Jeffrey T. Foster,James M. Schupp,John D. Gillece,Elizabeth M. Driebe,Cindy M. Liu,Cindy M. Liu,Burkhard Springer,Irena Zdovc,Antonio Battisti,Alessia Franco,Jacek Żmudzki,Stefan Schwarz,Patrick Butaye,Eric Jouy,Constança Pomba,M. Concepción Porrero,Raymond Ruimy,Tara C. Smith,D. Ashley Robinson,J. Scott Weese,Carmen Sofia Arriola,Fangyou Yu,Frédéric Laurent,Paul Keim,Paul Keim,Robert Skov,Frank Møller Aarestrup +34 more
TL;DR: The results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA, which appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance.
Journal ArticleDOI
Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark.
TL;DR: Differences in the occurrence of resistance and tetracycline resistance genes were observed among isolates from the different sources, however, similar resistance patterns and resistant genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs.
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