Identification of acquired antimicrobial resistance genes
Ea Zankari,Henrik Hasman,Salvatore Cosentino,Martin Vestergaard,Simon Rasmussen,Ole Lund,Frank Møller Aarestrup,Mette Voldby Larsen +7 more
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TLDR
A web server providing a convenient way of identifying acquired antimicrobial resistance genes in completely sequenced isolates was created, and the method was evaluated on WGS chromosomes and plasmids of 30 isolates.Abstract:
Objectives
Identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.read more
Citations
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Journal ArticleDOI
Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore.
Kar Hui Ong,Wei Ching Khor,Jing Yi Quek,Zi Xi Low,Sathish Arivalan,Mahathir Humaidi,Cliff Chua,Kelyn Lee Ghee Seow,Siyao Guo,Moon Y. F. Tay,Joergen Schlundt,Lee Ching Ng,Lee Ching Ng,Kyaw Thu Aung +13 more
TL;DR: Findings in this study suggested that wild birds and rodents could play a role in disseminating antimicrobial resistant E. coli, and this underscores the necessity of environment management and close monitoring on AMR bacteria in wild Birds and rodents to prevent spreading of resistant organisms to other wildlife animals and humans.
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Haemophilus ducreyi Cutaneous Ulcer Strains Are Nearly Identical to Class I Genital Ulcer Strains.
Dharanesh Gangaiah,Kristen M. Webb,Tricia L. Humphreys,Kate R. Fortney,Evelyn Toh,Albert K. Tai,Samantha S. Katz,Allan Pillay,Cheng-Yen Chen,Sally Roberts,Robert S. Munson,Stanley M. Spinola +11 more
TL;DR: These data suggest that CU strains are derivatives of class I strains that were not recognized until recently, and require confirmation by analysis of CU strains from other regions.
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Gene Loss and Acquisition in Lineages of Pseudomonas aeruginosa Evolving in Cystic Fibrosis Patient Airways.
TL;DR: The genomes of 45 Pseudomonas aeruginosa lineages evolving in the lungs of cystic fibrosis patients were analyzed to identify genes that are lost or acquired during the first years of infection and found that a notable proportion of such genes are associated with virulence; a trait previously shown to be important for adaptation.
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First environmental sample containing plasmid-mediated colistin-resistant ESBL-producing Escherichia coli detected in Norway.
Silje Bakken Jørgensen,Arne Søraas,Lotte P. Stenfors Arnesen,Truls Michael Leegaard,Arnfinn Sundsfjord,Pål A. Jenum +5 more
TL;DR: The detection of the plasmid borne mcr‐1 gene conferring colistin resistance in an extended‐spectrum β‐lactamase (ESBL) producing Escherichia coli ST10 strain retrieved from seawater at a public beach in Norway illustrates that E. coli strains carryingplasmid‐mediated colistIn resistance genes have also reached areas where this drug is hardly used at all.
Journal ArticleDOI
Genomic dynamics of species and mobile genetic elements in a prolonged blaIMP-4-associated carbapenemase outbreak in an Australian hospital.
A Kizny Gordon,H T T Phan,H T T Phan,Samuel Lipworth,Elaine Y. L. Cheong,Elaine Y. L. Cheong,Thomas Gottlieb,Thomas Gottlieb,Sophie George,Sophie George,T Peto,T Peto,Amy J. Mathers,Anne-Sophie Walker,Anne-Sophie Walker,D W Crook,D W Crook,Nicole Stoesser +17 more
TL;DR: Investigating the genomic dynamics of a 10 year outbreak of blaIMP-4-containing organisms in a burns unit in a hospital in Sydney, Australia found genetic backgrounds disseminating bla IMP- 4 can persist, diversify and evolve amongst both human and environmental reservoirs during a prolonged outbreak despite intensive prevention efforts.
References
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Journal ArticleDOI
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria
Mette Voldby Larsen,Salvatore Cosentino,Simon Rasmussen,Carsten Friis,Henrik Hasman,Rasmus L. Marvig,Lars Jelsbak,Thomas Sicheritz-Pontén,David W. Ussery,Frank Møller Aarestrup,Ole Lund +10 more
TL;DR: A Web-based method for MLST of 66 bacterial species based on whole-genome sequencing data that enables investigators to determine the sequence types of their isolates on the basis of WGS data.
Journal ArticleDOI
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.
TL;DR: A preliminary assessment of the amino acids which may be important in binding aminoglycosides was obtained from data and from the results of mutational analysis of several of the genes encoding am inoglycoside-modifying enzymes.
Journal ArticleDOI
Acquired Antibiotic Resistance Genes: An Overview
Angela H.A.M. van Hoek,Dik Mevius,Dik Mevius,Beatriz Guerra,Peter Mullany,Adam P. Roberts,Henk Aarts +6 more
TL;DR: Attention is paid to mobile genetic elements such as plasmids, transposons, and integrons, which are associated with AR genes, and involved in the dispersal of antimicrobial determinants between different bacteria.
Journal ArticleDOI
Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock
Lance B. Price,Marc Stegger,Henrik Hasman,Maliha Aziz,Jesper Larsen,Paal Skytt Andersen,Talima Pearson,Andrew E. Waters,Jeffrey T. Foster,James M. Schupp,John D. Gillece,Elizabeth M. Driebe,Cindy M. Liu,Cindy M. Liu,Burkhard Springer,Irena Zdovc,Antonio Battisti,Alessia Franco,Jacek Żmudzki,Stefan Schwarz,Patrick Butaye,Eric Jouy,Constança Pomba,M. Concepción Porrero,Raymond Ruimy,Tara C. Smith,D. Ashley Robinson,J. Scott Weese,Carmen Sofia Arriola,Fangyou Yu,Frédéric Laurent,Paul Keim,Paul Keim,Robert Skov,Frank Møller Aarestrup +34 more
TL;DR: The results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA, which appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance.
Journal ArticleDOI
Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark.
TL;DR: Differences in the occurrence of resistance and tetracycline resistance genes were observed among isolates from the different sources, however, similar resistance patterns and resistant genes were detected frequently indicating that transmission of resistant enterococci or resistance genes takes place between humans, broilers, and pigs.
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