Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
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Preventive Effect of Cocoa Flavonoids via Suppression of Oxidative Stress-Induced Apoptosis in Auditory Senescent Cells
Luz Del Mar Rivas-Chacón,Joaquin Yanes-Diaz,Beatriz de Lucas,Juan Riestra-Ayora,Raquel Madrid-García,Ricardo Sanz-Fernández,Carolina Sánchez-Rodríguez +6 more
TL;DR: In conclusion, CPE inhibits the activation of senescence-related apoptotic signaling by decreasing oxidative stress in auditory senescent cells and Administration of CPE also decreased oxidative DNA damage in the auditorysenescent cells.
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Polydopamine coated Au-Pt nanorods: Enhanced photothermal properties and efficient reactive oxygen scavengers.
Qianqian Duan,Карел Недбалек,Jialin Wang,Boye Zhang,Xiaoyuan Wang,Juanjuan Xue,Wendong Zhang,Shengbo Sang +7 more
TL;DR: In this paper, a dumbbell-shaped Au-Pt bimetallic nanorods (AuPtNRs) with biocompatible polydopamine (PDA) was used to obtain AuPt@PDA.
Journal ArticleDOI
Easy access of dihydrofluoresceins as advanced fluorescence turn-on probes for oxidative stress
Tao Deng,Huayu Bao,Wenyi Huang,Xiaojuan Wang,Shiyou Hu,Shengjun Wu,Liang Zhao,Chun Cai,Yingjie Hu,Fang Liu +9 more
TL;DR: In this article, a mild NABH4/I2 strategy was designed to easily reduce fluoresceins to dihydrofluorescein alcohols and acids, which showed great potentials for fluorescent imaging of subcellular oxidative stresses.
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Direct acupuncture of nitric oxide by an electrochemical microsensor with high time-space resolution
TL;DR: In this paper , an electrocatalytic microsensor was designed and fabricated for the direct detection of NO within the time-space resolution, which can be used to trace the release pattern of NO by different cancer cell lines and have known its dynamics in tumor microenvironment.
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Cytoprotective Effects of Cell-Permeable Bifunctional Antioxidant Enzyme, GST-TAT-SOD, against Cisplatin-Induced Cell Damage
TL;DR: The results suggest that GST-TAT-SOD might be a potential cytoprotective agent for cisplatin-induced side effects.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.