Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
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Heat shock induces the cellular antioxidant defenses peroxiredoxin, glutathione and glucose 6-phosphate dehydrogenase through Nrf2.
TL;DR: The results suggest that heat shock-generated ROS were involved in induction of cellular defense molecules Prxs, GSH and G6PD through Nrf2 activation, and sheds new light on the role of NRF2 and antioxidants in cellular responses to heat shock at mild and lethal temperatures.
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Implications for oxidative stress and astrocytes following 26S proteasomal depletion in mouse forebrain neurones.
Jamal Elkharaz,Aslihan Ugun-Klusek,Dumitru Constantin-Teodosiu,Karen Lawler,R. John Mayer,E. Ellen Billett,James Lowe,Lynn Bedford +7 more
TL;DR: This is the first in vivo report of oxidative stress caused directly by neuronal proteasome dysfunction in the mammalian brain, contributing to understanding neuronal-glial interactions in disease pathogenesis, providing an in vivo link between prominent disease hypotheses and are of relevance to a heterogeneous spectrum of neurodegenerative diseases.
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Antiproliferative and antimetabolic effects behind the anticancer property of fermented wheat germ extract
Christoph Otto,Theresa Hahlbrock,Kilian Eich,Ferdi Karaaslan,Constantin Jürgens,Christoph-Thomas Germer,Armin Wiegering,Ulrike Kämmerer +7 more
TL;DR: FWGE and the DMBQ compound both induced oxidative stress-promoted cytotoxicity and exhibited cytostatic and growth delay effects associated with impaired glucose utilization which led to autophagy, a possible previously unknown mechanism behind the influence of FWGE on cancer cell metabolism.
Journal ArticleDOI
Sinapine, but not sinapic acid, counteracts mitochondrial oxidative stress in cardiomyocytes
Doria Boulghobra,Pierre-Edouard Grillet,Mickaël Laguerre,Mathieu Tenon,Jérémy Fauconnier,Pascale Fança-Berthon,Cyril Reboul,Olivier Cazorla +7 more
TL;DR: Sinapine, a natural cationic hydrophilic phenol, commonly and substantially found in rapeseed species, effectively enters within the mitochondria, selectively decreases the level of mitochondrial oxidative stress and efficiently limits ROS production during cardiac ischemia-reperfusion.
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DNA damage in Fabry patients: An investigation of oxidative damage and repair.
Giovana Brondani Biancini,Dinara Jaqueline Moura,Paula R. Manini,Jéssica Lamberty Faverzani,Cristina Brinckmann Oliveira Netto,Marion Deon,Roberto Giugliani,Jenifer Saffi,Carmen Regla Vargas +8 more
TL;DR: Fabry patients presented efficient DNA repair in both assays as well as significantly higher levels of oxidative species (measured by dichlorofluorescein content), and even if DNA repair be induced in Fabry patients, the repair is not sufficient to reduce DNA damage to control levels.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.