Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
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Cationic liposomes evoke proinflammatory mediator release and neutrophil extracellular traps (NETs) toward human neutrophils
Tsong-Long Hwang,Ching-Yun Hsu,Ibrahim A. Aljuffali,Chun-Han Chen,Yuan-Ting Chang,Jia-You Fang,Jia-You Fang +6 more
TL;DR: This is the first report providing evidence of NET production induced by cationic liposomes, and it is important to consider the toxicity of cationo-liposomes during administration in the body.
Journal ArticleDOI
Synthesis and characterization of the anticancer and metal binding properties of novel pyrimidinylhydrazone derivatives.
Veronika F.S. Pape,Veronika F.S. Pape,Dóra Türk,Pál Szabó,Michael Wiese,Éva A. Enyedy,Gergely Szakács +6 more
TL;DR: The results demonstrate that the studied pyrimidinylhydrazones form redox-active iron and copper complexes that are capable of producing intracellular ROS, which might lead to cellular damage and cell death in cancer cells regardless of their resistance status.
Journal ArticleDOI
Neuronal Redox-Imbalance in Rett Syndrome Affects Mitochondria as Well as Cytosol, and Is Accompanied by Intensified Mitochondrial O 2 Consumption and ROS Release.
Karolina Can,Christiane Menzfeld,Lena Rinne,Peter Rehling,Sebastian Kügler,Gocha Golubiani,Gocha Golubiani,Jan Dudek,Michael Müller +8 more
TL;DR: Intensified mitochondrial O2 consumption, increased mitochondrial ROS generation and disturbed redox balance in mitochondria and cytosol may represent a causal chain, which provokes dysregulated proteins, oxidative tissue damage, and contributes to neuronal network dysfunction in RTT.
Journal ArticleDOI
Nitrite exerts antioxidant effects, inhibits the mTOR pathway and reverses hypertension-induced cardiac hypertrophy.
Danielle A. Guimaraes,Madla A. Passos,Elen Rizzi,Lucas C. Pinheiro,Jefferson H. Amaral,Raquel F. Gerlach,Michele M. Castro,Jose E. Tanus-Santos +7 more
TL;DR: Evidence is provided that nitrite treatment decreases cardiac remodeling induced by hypertension as a result of its antioxidant effects and downregulation of mTOR signaling pathway, which may help to establish nitrite as an effective therapy in hypertension‐induced cardiac hypertrophic remodeling.
Journal ArticleDOI
Redox biosensors in a context of multiparameter imaging.
Alexander I. Kostyuk,Anastasiya S. Panova,Dmitry S. Bilan,Vsevolod V. Belousov,Vsevolod V. Belousov +4 more
TL;DR: The main types of genetically encoded biosensors, the most frequently used readout, and their use in multiplexed imaging mode are described.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
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Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.