Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
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Challenges and advances in quantum dot fluorescent probes to detect reactive oxygen and nitrogen species: A review
TL;DR: This review highlights the challenges faced, pros and cons and published developments to date, with respect to QD fluorescent probes for ROS/RNS such as H2O2, O2(·-), ·OH, HOCl, NO and ONOO(-).
Journal ArticleDOI
Microstructure and antibacterial efficacy of graphene oxide nanocomposite fibres.
Rupy Kaur Matharu,Tanveer A. Tabish,Thithawat Trakoolwilaiwan,Jessica C. Mansfield,Julian Moger,Tongfei Wu,Claudio Lourenco,Biqiong Chen,Lena Ciric,Ivan P. Parkin,Mohan Edirisinghe +10 more
TL;DR: GO nanosheets embedded in a polymer matrix have demonstrated the ability to retain their antibacterial properties, thus offering themselves as a promising antibacterial agent.
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Reducing Mitochondrial ROS Improves Disease-related Pathology in a Mouse Model of Ataxia-telangiectasia
Anthony D. D’Souza,Ian A. Parish,Diane S. Krause,Susan M. Kaech,Susan M. Kaech,Gerald S. Shadel +5 more
TL;DR: Proof-of-principle that reducing mitochondrial ROS production per se may be therapeutic for the disease A-T is provided, which may have advantages compared with more general antioxidant strategies.
Journal ArticleDOI
Inactivation dynamics of 222 nm krypton-chlorine excilamp irradiation on Gram-positive and Gram-negative foodborne pathogenic bacteria.
TL;DR: It was found out that the KrCl excilamp induced cell membrane damage as a form of depolarization and from the study of respiratory chain dehydrogenase activity and the lipid peroxidation assay, it was revealed that cell membraneDamage was attributed to inactivation of enzymes related to generation of membrane potential and occurrence of lipid per oxidation.
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Increased miltefosine tolerance in clinical isolates of Leishmania donovani is associated with reduced drug accumulation, increased infectivity and resistance to oxidative stress.
Deepak Kumar Deep,Deepak Kumar Deep,Ruchi Singh,Vasundhra Bhandari,Aditya Verma,Vanila Sharma,Saima Wajid,Shyam Sundar,V. Ramesh,Jean-Claude Dujardin,Poonam Salotra +10 more
TL;DR: Genes associated with oxidative stress, metabolic processes and transporters showed modulated expression in LdRelapse and LdM30 parasites in comparison with LdPreTx parasites, indicating a better tolerance for oxidative stress in L dRelapse isolates.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.