Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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Action of Hydrogen Peroxide on Synaptic Transmission at the Mouse Neuromuscular Junction.
TL;DR: The effective inhibition of neurotransmitter release by ROS, which was proportional to the intensity of synaptic activity at the neuromuscular junction, was revealed and the combination of various oxidants suggested an intracellular location for redox-sensitive sites responsible for modulation of the synaptic transmission in the skeletal muscle.
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Potential nanomedicine applications of multifunctional carbon nanoparticles developed using green technology
TL;DR: This study focused on the preparation of carbon nanoparticles (CNPs) using edible yogurt drink (lassi) by microwave irradiation and demonstrated that CNPs had average size and doxorubicin was used as model molecule to evaluate sythesized CNPs for their efficacy in drug delivery.
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Phenolic extracts from whole wheat biofortified bread dampen overwhelming inflammatory response in human endothelial cells and monocytes: major role of VCAM-1 and CXCL-10
Nadia Calabriso,Marika Massaro,Egeria Scoditti,Antonella Pasqualone,Barbara Laddomada,Maria Annunziata Carluccio +5 more
TL;DR: The bread biofortified with selected durum wheat milling by-products is suggested as a source of phenolic acids with multiple anti-inflammatory and anti-atherosclerotic properties, which could help to counteract or prevent inflammatory vascular diseases.
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Blocking mitochondrial cyclophilin D ameliorates TSH-impaired defensive barrier of artery
Xiaojing Liu,Heng Du,Qiang Chai,Qing jia,Lu Liu,Meng Zhao,Jun Li,Hui Tang,Wenbin Chen,Lifang Zhao,Li Fang,Ling Gao,Jiajun Zhao +12 more
TL;DR: It is revealed that elevated TSH triggers mitochondrial perturbations in ECs and insights are provided that blocking mitochondrial CypD enhances the defensive ability of ECs under TSH exposure.
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Titania coating of mesoporous silica nanoparticles for improved biocompatibility and drug release within blood vessels.
TL;DR: It is demonstrated that titania coating of MSNs significantly improves their biocompatibility and uptake within aortic blood vessels and furthermore, enables a slower and more sustained release of the vasodilator drug, sodium nitroprusside within the vessel, thus making them an attractive strategy for the treatment of vascular disease.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
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ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
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Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
Journal Article
Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.