Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
Balaraman Kalyanaraman,Victor M. Darley-Usmar,Kelvin J.A. Davies,Phyllis A. Dennery,Henry Jay Forman,Henry Jay Forman,Matthew B. Grisham,Giovanni E. Mann,Kevin P. Moore,L. Jackson Roberts,Harry Ischiropoulos +10 more
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2012-01-01 and is currently open access. It has received 1423 citations till now.read more
Citations
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High-energy visible light at ambient doses and intensities induces oxidative stress of skin-Protective effects of the antioxidant and Nrf2 inducer Licochalcone A in vitro and in vivo.
Tobias Mann,Kerstin Eggers,Frank Rippke,Mirko Tesch,Anette Buerger,Maxim E. Darvin,Sabine Schanzer,Martina C. Meinke,Jürgen Lademann,Ludger Kolbe +9 more
TL;DR: Strong antioxidants are needed as additives to sunscreen products to prevent skin damage through the generation of reactive oxygen species (ROS).
Journal ArticleDOI
Puerarin protects dopaminergic neurons in Parkinson’s disease models
Xiaowei Zhang,Jing Xiong,Songyun Liu,Luyang Wang,Jinsha Huang,Ling Liu,Jiaolong Yang,Guoxin Zhang,Kexin Guo,Zhentao Zhang,Ping Wu,Dingan Wang,Zhicheng Lin,Nian Xiong,Tao Wang +14 more
TL;DR: Puerarin alleviated the oxidative stress and apoptosis in a PD cellular model, protected the dopaminergic neurons against rotenone toxicity and decreased the abnormal protein overexpressing in PD animal models, suggesting that puerarin may develop into a neuroprotective alternative for patients with PD.
Journal ArticleDOI
Blebbistatin, a myosin inhibitor, is phototoxic to human cancer cells under exposure to blue light
TL;DR: These findings offer new possibilities for blebbistatin as a potential anticancer and photodynamic agent and its reactive oxygen species (ROS) generating properties.
Journal ArticleDOI
Evaluating biomarkers to model cancer risk post cosmic ray exposure.
Deepa M. Sridharan,Aroumougame Asaithamby,Steve R. Blattnig,Sylvain V. Costes,Paul W. Doetsch,William S. Dynan,Philip Hahnfeldt,Lynn Hlatky,Yared H. Kidane,Amy Kronenberg,Mamta D. Naidu,Leif E. Peterson,Ianik Plante,Artem L. Ponomarev,Janapriya Saha,Antoine M. Snijders,Kalayarasan Srinivasan,Jonathan Tang,Erica Werner,Janice M. Pluth +19 more
TL;DR: The relationship of biomarkers to different potential cell fates, including proliferation, apoptosis, senescence, and loss of stemness, which can propagate genomic instability and alter tissue composition and the underlying mRNA signatures that contribute to cell fate decisions are discussed.
Journal ArticleDOI
Effects of natural phenolic compound carvacrol on the human gastric adenocarcinoma (AGS) cells in vitro.
Ayse Günes-Bayir,Huriye Senay Kiziltan,Abdurrahim Kocyigit,Eray Metin Guler,Ersin Karataş,Ali Toprak +5 more
TL;DR: There was a close negative relationship between cell viability and ROS level and carvacrol inhibited the proliferation of AGS cells, suggesting that it could be a novel and strong anticancer agent against the human gastric adenocarcinoma.
References
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Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis
TL;DR: The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling.
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Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.
TL;DR: Data suggest that H2O2-Fe(2+)-derived oxidant is mainly responsible for the nonenzymatic oxidation of DCFH, which remains an attractive probe as an overall index of oxidative stress in toxicological phenomena.
Journal ArticleDOI
Reconciling the chemistry and biology of reactive oxygen species
TL;DR: This review examines how target selectivity and antioxidant effectiveness vary for different oxidants and highlights areas where greater understanding is required on the fate of oxidants generated by cellular NADPH oxidases and on the identification of oxidant sensors in cell signaling.
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Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation.
David A. Bass,J W Parce,Lawrence R. DeChatelet,Pamela Szejda,Michael C. Seeds,Michael J. Thomas +5 more
TL;DR: The data indicate that the DCFH oxidation assay is quantitatively related to the oxidative metabolic burst of PMNL, and they strongly suggest that the reaction is mediated by H2O2 generated by the PMNL.